Raman Spectroscopic Analysis of Blood, Urine, Saliva and Tissue of Oral Potentially Malignant Disorders and Malignancy-A Diagnostic Study
Journal Title: International Journal of Oral and Craniofacial Science - Year 2016, Vol 2, Issue 1
Abstract
Background: Oral cancers are mostly being preceded by oral potentially malignant disorders (OPMD). Early diagnosis of oral cancer or OPMDs speed up treatment and improve prognosis. Raman spectroscopy of blood, urine, saliva and tissue samples collected from OPMD and oral cancer patients were compared with similar samples from normal controls. Context and purpose: Raman spectroscopy is a noninvasive inelastic light scattering technique in which the wavelength of the incident laser light shifts depending on the vibrations of the molecules. The specific biochemical, structural and conformational changes occurring in tissues is reflected by their Raman spectra well before the clinical manifestations start. This helps in early diagnosis and speedy treatment planning. Results: Raman spectroscopy gave an accuracy of 78%, 90.5%, 93.1%, and 97.4% respectively for blood, urine, saliva and tissue samples in discriminating oral pre malignancy and malignancy from normal control. Conclusion: Results of the study validate that Raman spectroscopy has the potential to be a diagnostically useful tool for the in vitro detection of OPMDs and oral cancers at the molecular level. Brief Summary: Early diagnosis of OPMD and oral cancer by analyzing Raman spectroscopic changes improves the prognosis. Total sample size was 205. Spectra recorded on a confocal Micro Raman System (LABRAM HR 800) were statistically analyzed using the computer software SPSS/PC +19 under one of the multivariant technique analysis - Principal Component analysis followed with the Linear Discriminant Analysis (PC-LDA). The statistical analysis using PC-LDA combination of normal vs malignant vs premalignant group accuracy are shown.
Authors and Affiliations
Jaychandran S, Meenapriya PK, Ganesan S
Keywords
Related Articles
- EP ID EP335958
- DOI 10.17352/2455-4634.000013
- Views 95
- Downloads 0
A Comparative Study of the Efficiency of two Different Glass Ionomer Using ART Technique in a Group of Egyptian Children with Autistic Spectrum Disorder
Aim: The aim of the study was to evaluate and compare the effect of conventional glass ionomer cement and Nano-glass ionomer using atraumatic restorative technique (ART) in a group of Egyptian autistic children. Materia...
A Unique Case: Solitary Fibrous Tumor of the Mandible
Solitary fibrous tumors (SFTs) were firstly reported in the pleura by Klemperer and Rabin [1], in 1931. These tumors are rare lesions and most commonly arise in the thoracic cavity [2]. Most of them occur as slow-growing...
Mass Dimension Evaluation of the Optic Nerve Head Microvascularity in Non-Glaucomatous Optic Neuropathies
In several diseases, fractal analysis has been proven useful to give contribution in diagnosis and prognosis of the patient and to improve treatments. In the present work we have evaluated the mass dimension of the vascu...
Raman Spectroscopic Analysis of Blood, Urine, Saliva and Tissue of Oral Potentially Malignant Disorders and Malignancy-A Diagnostic Study
Background: Oral cancers are mostly being preceded by oral potentially malignant disorders (OPMD). Early diagnosis of oral cancer or OPMDs speed up treatment and improve prognosis. Raman spectroscopy of blood, urine, sal...
Sentinel Lymph Node Biopsy vs. Elective Neck Dissection in Patients with T1/T2 N0 Oral Squamous Cell Carcinoma: A Matched Pair Analysis
Background: The management of the clinically and radiological negative neck in patients with early squamous cell carcinoma of head and neck (HNSCC) is still controversial. As approximately 20 to 30% of these patients har...