RAPID IN VITRO CALLOGENESIS AND PHYTOCHEMICAL SCREENING OF LEAF, STEM AND LEAF CALLUS OF MUSSAENDA FRONDOSA LINN. - A MEDICINAL PLANT.
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2017, Vol 10, Issue 6
Abstract
Objective: To standardise the protocol for rapid callogenesis in Mussaenda frondosa L. using leaf explants. Qualitative and quantitative phytochemical analysis of leaf, stem and callus cultures.Methods: The leaf explants were inoculated onto MS medium supplemented with varying concentrations of growth regulators such as 2, 4 - D, NAA, BAP, Kn for the induction of callus. Qualitative and quantitative analysis of total phenol, flavonoids and alkaloids contents of leaf, stem and callus were tested by standard methods. The antioxidant activities were investigated using DPPH radical scavenging method and reducing power assay. The anti - inflammatory activity was evaluated by membrane stabilizing activity.Results: Pale green, healthy, friable and fast growing callus was obtained on the medium enriched with NAA (2mg/l) + Kn (4mg/l). Quantitative determination showed the highest concentration of total phenolics in the methanolic extract of in vitro grown callus (10 ± 1.1 mg of GA/g of extract), flavonoids in methanolic stem extract (137±1.6 mg of Quercitin/g of extract) and alkaloids in methanolic extract of leaf (118.3±1.5 mg/10g of extract). The methanolic leaf extract exhibited highest free radical scavenging activity with IC50 value of 40.6±10.06 μg/ml. The highest membrane stabilizing activity was shown by chloroform extract of the leaf (66.02%).Conclusion: The present preliminary phytochemical and pharmacological analysis may form the basis for drug development in future using callus cultures of M. frondosa.  Â
Authors and Affiliations
Manasa Dj, Chandrashekar Kr, Bhagya N
Keywords
Related Articles
- EP ID EP605302
- DOI 10.22159/ajpcr.2017.v10i6.17527
- Views 123
- Downloads 0
A CROSS-SECTIONAL STUDY ON THE PREVALANCE AND RISK FACTORS OF LYMPHOEDEMA IN CARCINOMA BREAST
Objectives: Lymphedema of the arm is a devastating complication of breast carcinoma treatment. There is a lack of research on the risk factors and methods of preventing upper limb lymphedema after breast carcinoma treatm...
VALIDATED RP-HPLC METHOD AND UNIQUE MOBILE PHASE FOR THE SIMULTANEOUS ESTIMATION OF AMLODIPINE BESYLATE AND VALSARTAN FROM SOLID DOSAGE FORM AND ROSUVASTATIN AND VALSARTAN FROM BULK
Objective: A reverse-phase high-performance liquid chromatographic (RP-HPLC) method was developed and validated for the simultaneous estimation of amlodipine besylate (AD) and valsartan (VAL) in pharmaceutical dosage for...
EVALUATION EVALUATION OF PHYTOCHEMICAL COMPOUNDS AND ANTIMICROBIAL ACTIVITIES OF HEMIDESMUS INDICUS
Objective: The present study was aimed to investigate the phytochemical screening, antibacterial activities, and identification of bioactive compounds by gas chromatography-mass spectrometry (GC-MS) and high-performance...
DRUG UTILIZATION ANALYSIS OF ANTI-SNAKE VENOM AT A TERTIARY CARE CENTER IN CENTRAL MAHARASHTRA: A 3-YEAR RETROSPECTIVE STUDY
Objectives: In India, snakebite is a major public health problem. Anti-snake venom serum (ASVS) is the definitive treatment for poisonous snakebites. However, the non-availability of effective ASVS to treat the specific...
IN VITRO MASS PROPAGATION AND SYNTHETIC SEED PRODUCTION COMBINED WITH PHYTOCHEMICAL AND ANTIOXIDANT ANALYSIS OF BACOPA CHAMAEDRYOIDES: AN ETHNO-MEDICINALLY IMPORTANT PLANT
Objective: This study was carried out to optimize adventitious shoot buds induction from the surface of the leaf and internode explants along withinvestigation of the phytochemical constituents and in vitro antioxidant p...