RAPID SEPARATION AND ESTIMATION OF LAMIVUDINE AND ZIDOVUDINE IN MARKETED FORMULATIONS USING RP-HPLC METHOD AND ITS VALIDATION
Journal Title: Int J of Pharm Res & Analy - Year 2014, Vol 4, Issue 8
Abstract
The goal of the present investigation was carried out to develop a validated analytical RP-HPLC method for simultaneous separation and estimation of Lamivudine and Zidovudine for pharmaceutical formulations. The chromatographic separation was accomplished on Welchrom RP-C18 Column (250 mm X 4.6 mm; 5μm), Shimadzu LC-20AT Prominence Liquid Chromatograph and with a mixture of 10 mM Phosphate buffer (pH 3.6): acetonitrile (50:50, v/v). The flow rate was fixed at 1.2 mL/minute and the analysis was performed using Shimadzu SPD-20A Prominence UV-Visible detector at 241 nm. The Lamivudine and Zidovudine were separated within three minutes. The retention time for Lamivudine and Zidovudine was found to be 2.393 minutes and 2.7 minutes respectively. The calibration plots were linear over the concentration range of 2-10 μg/mL for Lamivudine (r2=0.9995) and 4-20 μg/mL for Zidovudine (r2=0.9991). There was no interference due to commonly used excipients. The % RSD for both the drugs were calculated and showed less than 2% which obviously indicates that the present method was said to be highly precise. Regarding accuracy of the developed method the %RSD were also found less than 2% which shows the method is completely accurate. The method was very sensitive with regard to limit of detection and limit of quantitation for Lamivudine and Zidovudine were found to be 0.181 μg/mL, 0.576 μg/mL and 0.551 μg/mL, 1.747μg/mL respectively. The mean assay values for Lamivudine and Zidovudine were arrived at 98.88% and 99.70 % respectively. The developed RP HPLC method was found to be simple, rapid, sensitive, highly precise and accurate and can be successfully employed for simultaneous estimation of Lamivudine and Zidovudine in pharmaceutical formulations
Authors and Affiliations
P. Ravisankar , G. Mounika , P. Srinivasa Babu
Keywords
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- EP ID EP90327
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