Ratio of the main phylotypes of gut microbiota in patients with non-alcoholic fatty liver disease and obesity
Journal Title: Сучасна гастроентерологія - Year 2019, Vol 0, Issue 5
Abstract
Objective — to study composition of the main phylotypes of the gut microbiota Bacteroidetes, Firmicutes and Actinobacteria and their ratio Firmicutes/Bacteroidetes in patients with non-alcoholic fatty liver disease (NAFLD) against the background of obesity depending on gender and age. Materials and methods. The study included 59 patients with NAFLD against the obesity background (the main group), from them 24 (40.7 %) women and 35 (59.3 %) men aged 43 to 62 years (median 49 years). The body mass index (BMI) was 35.60 (31.80; 39.30) kg/m2. The comparison group included 14 patients with NAFLD: 10 (71.4 %) women and 4 (28.6 %) men aged 36 to 58 years (median 51years) with and without obesity, the BMI 27.30 (27.00; 28.20) kg/m2. The control group consisted of 9 practically healthy volunteers, The groups were age- and gender-matching. All patients underwent an ultrasound examination of the liver and a general clinical laboratory examination, which additionally included measuring the ratio of the phylotype Bacteroidetes, Firmicutes and Actinobacteria in fecal content by real-time polymerase chain reaction with the calculation of the Firmicutes/Bacteroidetes ratio. Results. Significant changes in anthropometric parameters were observed in patients with NAFLD and obesity: an increase in BMI of 30.40 % (p < 0.05) and an increase in waist circumference by 25.00 % (p < 0.05). A decrease in the relative Bacteroidetes content was detected in patients with NAFLD with concomitant obesity and isolated NAFLD (14.74 (6.46; 28.80) % and 16.85 (8.46; 33.33) %, respectively) when compared with the control group (33.3 (8.48; 50.39) %) (H = 2.90, p = 0.23). The highest relative Firmicutes content was determined in patients with NAFLD (52.52 (42.39; 62.88) %) and in patients with comorbid course of NAFLD and obesity (51.35 (37.65; 67.80) %) and decrease in individuals of the main group at 32.97 (22.02; 43.08) %. This dynamics of the indicator was also preserved for the Firmicutes/Bacteroidetes ratio. In control group, the relative Actinobacteria content was 1.73 (1.16; 2.17) %, and in patients with NAFLD — up to 3.98 (1.53; 5.22) %, reaching maximum values in patients with concomitant obesity — 4.40 (2.54; 9.60) %. Conclusions. The ratio of the major intestinal microbiota phylotypes was significantly different in NAFLD patients, in particular in patients with concomitant obesity. In comparison with the group of healthy volunteers, the increase of the relative Actinobacteria content in 2.3 times and Firmicutes in 1.59 times was observed in patients with an isolated NAFLD course; in case of NAFLD and obesity comorbid course, the relative amount the increase was in 2.54 times for Actinobacteria, and in 1.55 times for Firmicutes level.
Authors and Affiliations
N. I. Chereliuk
Keywords
Related Articles
- EP ID EP675098
- DOI 10.30978/MG-2019-5-26
- Views 124
- Downloads 0
Modern approaches to the treatment of combed non-alcoholic fatty liver disease and cardiovascular disease
Non-alcoholic fatty liver disease (NAFLD) is a major cause of chronic liver disease and it progresses from simple steatosis to steatohepatitis, fibrosis and cirrhosis. It has been proven that NAFLD is a multisystem disea...
The gastroenterological manifestations of methotrexate effects in pediatric patients with the juvenile idiopathic arthritis
The paper elucidates the most prevalent gastroenterological manifestations of methotrexate effects in the treatment of juvenile idiopathic arthritis (JIA) in pediatric patients. Some considerable changes took place in th...
Helicobacter pylori infection and acid-related diseases against the background of metabolic-associated conditions: mechanisms of development and management
The author presents data on the role of H. pylori infection in the initiation and progression of the changes in metabolic status of patients with type 2 diabetes mellitus (DM-2), insulin resistance, obesity, cardiovascul...
The modern paradigm of the chronic gastritis, associated with bile reflux
The authors considered the modern concepts of the mechanisms of formation and progression of chronic gastritis associated with bile reflux. The main factors responsible for the onset of duodenogastric reflux have been el...
Predictors of irritable bowel syndrome formation among students
Objective — to establish the role of the nervous system disorders in the formation of irritable bowel syndrome (IBS) among students. Materials and methods. Examination involved 32 students with IBS, aged 19 to 26 years....