Research progress of gene editing technology CRISPR/Cas9 system in animal gene editing
Journal Title: International Journal of Veterinary Science and Research - Year 2018, Vol 4, Issue 1
Abstract
Gene editing technology, from the beginning of RNA interference (RNAi) technology to efficient developed enzyme technology, has been widely used in recent years. These efficient enzyme technologies include zinc finger nuclease (ZFN) technology, transcriptional activation-like effector nuclease (TALENs) technology, and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) 9 system (CRISPR/Cas9) technology. The CRISPR/Cas (Cas) system is a gene editing tool for DNA modification regulated by a short RNA and is a new type of genome editing tool that is faster, more efficient, and more accurate than the zinc finger nuclease and transcription activator-like effector nuclease. This article reviews the structure and function of the CRISPR/Cas system, and is aimed to outline the Cas9 design strategy, factors that affect the Cas9 gene editing efficiency, off-target detection and analysis methods, and especially the application in animal gene editing studies. Based on CRISPR/Cas9 gene editing has been successfully implemented in a variety of animals, and it is expected to become a new feasible way to establish animal models and study disease prevention in veterinary science and research.
Authors and Affiliations
Fan Feng, Mengna Kang, Xiaolan Zhu, Weiping Cao, Yueqin Liu, Qi Chen, Fangfang Lin, Yanwen Qianfu, Wenlina Xu
Keywords
Related Articles
- EP ID EP554207
- DOI 10.17352/ijvsr.000030
- Views 67
- Downloads 0
Freshwater Snail Distribution Related to Physicochemical Parameters and Aquatic Macrophytes in Giza and Kafr El-Shiekh Governorates, Egypt
A field work was conducted to study the density of the freshwater snail in relation to the vegetation cover as well as the physical and chemical properties in different watercourses. Two sites were selected in Giza gover...
ACTH Level and Sodium-Potassium Ratio in Screening of Primary Canine Hypoadrenocorticism
This article describes a primary hypoadrenocorticism (HA) case report in a spayed mix-breeded German shepherd ten years old female dog that had unspecific symptoms during a week. The initial suspicious were poisoning and...
Factors Affecting Lethality of Bisphenol a on Biomphalaria alexandrina Snails
The present study was undertaken to investigate the influence of some environmental factors including temperature, water vegetation, bed mud and pH on LC50, LC90and LT50 of bisphenol A (BPA) on the snail host of Schistos...
Review on Practical Guidance of Veterinary Clinical Diagnostic Approach
Animal disease problems have been investigated by veterinarians through undertaking a careful and clinical examination with the objective of recognizing the nature of the affection. So, that effective treatment and contr...
Mesenchymal stem cells from the domestic ungulates: trends and outliers
Mesenchymal stem cells (MSCs) are a valuable source for regenerative therapy and tissue engineering. MSCs are multipotent adherent stem cells that can be isolated from different adult and fetal tissues. In contrast to hu...