Rickettsia rickettsii isolation from naturally infected Amblyomma parvum ticks by centrifugation in a 24-well culture plate technique
Journal Title: Open Veterinary Journal - Year 2013, Vol 3, Issue 2
Abstract
Rocky Mountain spotted fever is an acute illness caused by Rickettsia rickettsii (R. rickettsii) and is transmitted by the bite of ticks of the genera Dermacentor, Amblyomma and Rhipicephalus. The illness results in a high mortality rate and may be easily confused with other febrile syndromes. In Yucatan State, Mexico, childhood cases with a high mortality have been reported. In this work we report the isolation of a Mexican R. rickettsii strain from a tick egg mass using an alternative method for Rickettsia isolation with 24-well plates. We also identified a potential vector of R. rickettsii in the southeast of Mexico, which is Amblyomma parvum.
Authors and Affiliations
K. Dzul-Rosado| Regional Research Center “Dr. Hideyo Noguchi”, Autonomous University of Yucatan, Mexico, G. Peniche-Lara| Faculty of Medicine, Interinstitutional Unit of Clinical and Epidemiological Research. Autonomous University of Yucatan, Mexico, R. Tello-Martín| Regional Research Center “Dr. Hideyo Noguchi”, Autonomous University of Yucatan, Mexico, J. Zavala-Velázquez| Faculty of Medicine, Interinstitutional Unit of Clinical and Epidemiological Research. Autonomous University of Yucatan, Mexico, R. de Campos Pacheco| Federal University of Mato Grosso, Laboratory of Veterinary Parasitology, Department of Basic Science and animal production, Faculty of Agronomy, Animal Medicine and zootechny, Brazil, M. B. Labruna| Parasitic Disease laboratory, Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Zootechny, São Paulo University, São Paulo, SP, Brazil, E. C. Sánchez| Government Health Services, Hospital Agustín O´Horan SSY, Calle Itzaes s/n. Col Centro, Merida Yucatan, Mexico, J. Zavala-Castro| Regional Research Center “Dr. Hideyo Noguchi”, Autonomous University of Yucatan, Mexico
Keywords
Related Articles
- EP ID EP9289
- DOI -
- Views 442
- Downloads 26
Arthroscopy of septic carpitis in donkeys (Equus asinus)
Experimental septic arthritis was induced in the radiocarpal joint of 18 donkeys by intra-articular injection of Staphylococcus aureus (3-4X106 CFU). The inoculated animals were divided into three groups (6 donkeys in e...
Glucosamine and chondroitin use in canines for osteoarthritis: A review
Osteoarthritis is a slowly progressive and debilitating disease that affects canines of all breeds. Pain and decreased mobility resulting from osteoarthritis often have a negative impact on the affected canine’s quali...
TGF-β1 serum concentrations and receptor expressions in the lens capsular of dogs with diabetes mellitus
Tissue fibrosis as complication of diabetes mellitus is known in humans. Because TGF-β1induces fibrosis and is elevated in humans suffering from diabetes mellitus we measured this growth factor in serum of dogs with di...
Histopathological changes in the head kidney induced by cadmium in a neotropical fish Colossoma macropomum
We evaluated the effect of cadmium (Cd) on the structure and function of the head kidney in the freshwater fish Colossoma macropomum (C. macropomum). Juveniles were exposed to 0.1 mg/L CdCl2 for 31 days. Blood samples...
Ovine paratuberculosis: a confirmed case of Johne's disease in Libya
Paratuberculosis (Johne's disease) was suspected in a herd of approximately 033 sheep after weight loss and scouring had increased in adult animals despite repeated treatment with anthelmintics, antibiotics, multivitami...