Role Of Dried Blood Spot In Early Infant Diagnosis Of HIV Infection
Journal Title: National Journal of Integrated Research in Medicine - Year 2012, Vol 3, Issue 1
Abstract
Background: Routinely the test that we use in HIV screening are HIV rapid test or Enzyme Immuno Assay (EIA), these methods can’t accurately diagnose HIV infection in infants up to the age of 18 months. Only way to confirm diagnosis is detection of HIV DNA from the children by nuclear techniques like polymerase chain reaction (PCR), which is not available at the resource limited laboratories. The technique of Dried Blood Spot (DBS) is a simple effective method of sample collection & transport for HIV testing of children below 18 months, especially at resource limited laboratories, which is the part of PPTCT programme. Method : The study group consists of 87 infants born to HIV reactive mothers under the PPTCT programme, at tertiary care center in Ahmedabad, Central Gujarat, India over a period of one year, from May-2010 to April 2011. Earliest DBS collection is recommended at the age of 6 week. Results: Total 87 children were included in the study. Total prevalence of HIV infection in children below 18 month of age is 8%. 2(12.5%) children born to mothers who had not received Nevirapine were tested positive for HIV by DNA PCR. 3 (20%) children became infected with HIV who had not received syrup Nevirapine. Rate of HIV infection in children who were breast fed is 4 (12.5%). 2 (20 %) mothers with CD4+ count less than 200/mm3 transmitted HIV infection to their babies. Conclusion: Ninety percent of the children living with HIV were infected through mother-to-child transmission during pregnancy, around the time of birth or through breast feeding. The technique of Dried Blood Spot (DBS) especially at resource limited laboratories & detection of HIV infection through the most sensitive and specific method PCR in diagnosis of HIV at earliest age, six weeks. Different interventions can be taken to prevent mother to child transmission during pre, intra and postnatal periods. [Dharsandia M et al NJIRM 2012; 3(1) : 85-89].
Authors and Affiliations
Dharsandia MV, Mangukiya JD, Mistry KN, Soni ST, Vegad MM
Keywords
Related Articles
- EP ID EP40191
- DOI -
- Views 377
- Downloads 0
A Study of Psychometric Properties Of Modified Rivermead Mobility Index
Background : To evaluate the effect treatment on mobility various studies have used. But responsiveness of Rivermaid mobility Index has found to be poor as items are scored on a dichotomous (yes/no) basis. MRMI is a mo...
A Study Of Supraorbital Notches And Foramina In Gujarati Human Skulls
The anatomy of the supraorbital notches and foramina has been studied in 249 human skulls. Of 233 skulls, 35.62% had bilateral supraorbital notches, 21.45% had bilateral supraorbital foramina and 16.73% had a notch on o...
Pancreaticoduodenectomy With Left Lateral Hepatic Segmentectomy : A Rare Procedure For The Management Of Complex Pancreaticoduodenal And Liver Injuries In Blunt Abdominal Trauma
Context : Pancreaticoduodenal complex injuries are uncommon after blunt abdominal trauma and are difficult to diagnose. Case Report : We report a case of 25 years old male who suffered massive Pancreaticoduodenal compl...
Comparison of Germ Tube Production By Candida Albicans In Various Media
Introduction: Candidiasis is one of the important opportunistic fungal infections in human. Candida albicans (C.albicans) is the predominant Candida species isolated from clinical samples. In routine laboratory, C.albi...
A large incidental adrenal mass in a patient with sudden onset multiple organ failure
A 55-year-old female was brought to the emergency room for sudden-onset chest pain and shortness of breath. Her only past medical history was mild hypertension not requiring medications. She had no family history of cor...