Role of Real Time PCR in Detection of MTB, MDR and XDR TB Directly from Sputum Specimens of Clinical Suspects of PTB
Journal Title: Journal of Medical Science And clinical Research - Year 2017, Vol 5, Issue 11
Abstract
Background and Objectives: “Presently, Sikkim has emerged as the next big concern on India’s already riddled tuberculosis map with 11 per cent of the new tuberculosis cases in the State found to be multi-drugresistant”; as reported in The Hindu; 14th July 2016.The study intended in verifying the role of real time PCR in the detection of Mycobacterium tuberculosis, diagnosis of multi drug resistant tuberculosis and extensively drug resistant tuberculosis directly in sputum samples of pulmonary tuberculosis. Materials and Methods: The sputum samples were subjected to fluorescence staining and real time PCR. For the detection of MDR and XDR TB cases the genes targeted were rpoB- rifampicin resistance, katG and in hA- isoniazid resistance, gyrA-fluoroquinolone resistance, rrs, eia-10 and eia-37- resistance to injectable drugs. Results: The study revealed 50(61%) sputum positive and 32 (39%) sputum negative cases. However, real time PCR diagnosed 64(78%) positive cases. Fourteen MDR TB and 1 XDR TB cases were diagnosed simultaneously, with maximum MDR TB cases belonging to 3+ AFB grading. Interpretation and Conclusion: Real time PCR showed better sensitivity, specificity and an early means of detection of Mycobacterium tuberculosisas well as multi drug resistant tuberculosis and extensively drug resistant tuberculosis in the suspected samples.
Authors and Affiliations
Preety Choudhary
Keywords
Related Articles
- EP ID EP525692
- DOI -
- Views 75
- Downloads 0
To Compare the Efficacy & Safety of Nerve Stimulation Method with Ultrasound Guidance along With Nerve Stimulation alone for Supraclavicular Brachial Plexus Block
There are several well-established techniques for performing peripheral nerve blocks including paresthesia, nerve stimulation and by using ultrasound. Those advocating the use of nerve stimulators claim that apart from a...
Correlation between End-Tidal Carbon Dioxide Pressure and Arterial Carbon Dioxide Partial Pressure in Patients Undergoing Craniotomy
Background: End-tidal carbon dioxide pressure (ETCO2) is commonly used as an indicator of arterial partial pressure of carbon dioxide (PaCO2) which reflects adequacy of ventilation during major surgeries. While accurate...
Assessment of Laboratory Practitioner’s Competency in Kenya
Introduction: There has been increasing interest to improve the quality of laboratory services world- wide including in many resource poor countries. In tandem with this need, the Kenya government recognizes the delivery...
Intraoperative Findings in Patients of Peptic Ulcer Perforation: A Prospective Study in Correlation with Patient Outcome
Background: Peptic ulcer perforation is an emergency and requires prompt diagnosis and urgent surgical intervention. Overall, peptic ulcer mortality and hospitalization rates have declined for the past two decades, but c...
Psychiatric Co-Morbidity in Patients with Attempted Suicide- A Hospital Based Study
Suicide is fatal act that represents the person’s wish to die. There is a range, however, between thinking about suicide and acting it out. Some persons have ideas of suicide that they will never act on. In all countries...