Roles of Toll-like Receptor 3 in Intrauterine Transmission of Hepatitis B Virus in Mothers with High Viral Load
Journal Title: Diseases & Research - Year 2021, Vol 1, Issue 1
Abstract
Backgrounds Despite passive and active immunization, perinatal mother-to-infant transmission (MTIT) of hepatitis B virus (HBV) still occurs in women with high levels of viremia. Thus, understanding the mechanisms of MTIT is essential to prevent MTIT. The aims of this study were to clarify the roles of toll-like receptor 3 (TLR3) in the prevention of hepatitis B transmission in mothers with a high viral load. Methods Placental samples were collected from 87 HBV-positive pregnant women and 25 normal pregnant women. Choriocarcinoma JEG-3 cell lines were exposed to different HBV viral loads to mimic the trophoblast barrier affected by HBV in the placenta. The mRNA and protein expression levels of TLR3 were analyzed by qRT-PCR and western blotting assays in placenta and JEG-3 cells, respectively. Results In terms of mRNA and protein expression, the expression of TLR3 in the placenta among the control, low viral load, medium viral load and high viral load groups were significantly different, showing significant upregulation in the medium load and high load groups compared with the control; TLR3 expression in the placenta of the HBeAg-positive group was higher than that in the HBeAg-negative group, and TLR3 expression in the placenta of the infant-infected group was lower than that of the infant-noninfected group. Expression of TLR3 was gradually increased in JEG-3 cells exposed to low HBV viral loads or with shortterm HBV exposure and was decreased in JEG-3 cells exposed to high HBV viral loads or with longterm HBV exposure. Conclusions TLR3 contribute to HBV intrauterine infection in mothers with a high viral load and, importantly, prevents mother-to-infant transmission.
Authors and Affiliations
Hong Gao, Ling Xu, Xiangying Zhang, Zhihao Fan, Hua Zhang, Zhongping Duan, Feng Ren
Keywords
Related Articles
- EP ID EP740190
- DOI 10.54457/DR.202101002
- Views 75
- Downloads 1
Effect of Comparison between Qingchangligan Granules and Qingchangligan Decoction on D-GalN/LPS-Induced Liver Injury in Mice
Ethnopharmacological relevance The Qingchangligan formula has two dosage forms, granules and decoction. Both of them have the same prescription; however, whether their protective effects are different remain elusive. Ai...
Mechanical Large Bowel Obstruction as the Initial Presentation of Locally Advanced Pancreatic Tail Carcinoma: A Case Report and Literature Review
We report a rare case of large bowel obstruction caused by locally advanced pancreatic tail adenocarcinoma. A 58-year-old male chronic smoker presented with progressive abdominal distention and absolute constipation for...
Secretome of Dental Pulp-Derived Stem Cells Reduces Inflammation and Proliferation of Glioblastoma Cells by Deactivating Mapk-Akt Pathway
Background Dental pulp-derived stem cells (DPSC) is a promising therapy as they modulate the immune response, so we evaluated the inhibitory effect of DPSC secretome (DPSC℗) on the proliferation and inflammation in human...
Roles of Toll-like Receptor 3 in Intrauterine Transmission of Hepatitis B Virus in Mothers with High Viral Load
Backgrounds Despite passive and active immunization, perinatal mother-to-infant transmission (MTIT) of hepatitis B virus (HBV) still occurs in women with high levels of viremia. Thus, understanding the mechanisms of MTIT...
Bioinformatics Analysis to Screen the Key Genes and Pathways in the Formation of Early T-cell Precursor Acute Lymphoblastic Leukemia
Purpose Early T-cell precursor acute lymphoblastic leukemia (EPT-ALL) is a rare type of ALL that shows genetic characteristics of both ALL and acute myeloid leukemia (AML) but has a poorer prognosis and a higher recurren...