RP-HPLC assay method development and validation of ketorolac and olopatidine in opthalmic liquid dosage forms
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2014, Vol 3, Issue 5
Abstract
A simple reverse phase high performance liquid chromatographic assay method was developed and validated for the simultaneous estimation of Ketorolac and olopatadine in ophthalmic liquid dosage forms. The chromatographic separation was achieved on phenomenex C18 (250 mm × 4.6 mm id, 5 μm particle size) column by using the mobile phase composition of ammonium acetate: acetonitrile: methanol buffer (50:30:20 v/v, pH 5.5), the detection of analyte was done at 212 nm for ketorolac and olopatadine respectively by PDA detector. LOD was found to be 0.78μg/ml and 0.45μg/ml and LOQ was found to be 2.36μg/ml and 1.37μg/ml for ketorolac and olopatadine respectively.
Authors and Affiliations
B. Haseena Banu*| Department of Pharmaceutical Analysis & Pharmaceutical Chemistry, Krishna Teja Pharmacy College, Chadalawada Nagar, Renigunta Road, Tirupati-517502, Andhra Pradesh, India, and Battala Priyanka| Department of Pharmaceutical Analysis & Pharmaceutical Chemistry, Krishna Teja Pharmacy College, Chadalawada Nagar, Renigunta Road, Tirupati-517502, Andhra Pradesh, India
Keywords
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