SCREENING AND OPTIMIZATION OF EXTRACELLULAR AMYLASE PRODUCTION FROM PLANT GROWTH PROMOTING RHIZOBACTERIA
Journal Title: Annals. Food Science and Technology - Year 2011, Vol 12, Issue 2
Abstract
Total 50 Fluorescent Pseudomonas was screened for their ability to produce extracellular alpha-amylase and fifteen were selected for further studies due to amylolytic activity on starch agar plates. Of these 50 isolates, Pseudomonas fluorescence APK10 was selected for the further studies due to its higher amylase activity levels. P. fluorescence APK10 showed amylase activity at a wide range of pH (5.0 to 9.0) and temperature (20ºC to 40ºC). The optimum incubation time for amylase production was recorded as 24hrs (1050U/ml/min), while no activity detected after 96hrs of incubation. Higher alpha-amylase production was recorded at pH 7.0 (1500U/ml/min) and 30ºC (2000U/ml/min). P. fluorescence APK10 showed varied levels of enzyme activity with different starch concentrations. 2.0% starch concentration was the optimum concentration for maximum enzyme production (2500U/ml/min), while higher concentration above 2.5% reduced the enzyme activity. Alpha-amylase production varied greatly with different carbon source. Amongst the sugars, fructose, maltose, glycerol and trehalose encouraged maximum amylase production in comparison to starch, lactose, sucrose, glucose, mannitol, arabinose and xylose. While ribitol, raffinose, and sorbitol based medium was showed no or negligible enzyme activity. Results of this study revealed that isolated amylase was considered to be thermostable and active in a wide range of pH.
Authors and Affiliations
Arun Karnwal
Keywords
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