Screening and optimization of indole 3 acetic acid producing non-heterocystous cyanobacteria isolated from saline soil
Journal Title: Scholars Academic Journal of Biosciences - Year 2016, Vol 4, Issue 9
Abstract
Cyanobacteria are diverse group of oxygenic photosynthetic prokaryotes. Indole 3 acetic acid is one of the major phytohormones for plant growth. To explore the Indole 3 Acetic Acid (IAA) production by cyanobacteria from saline soil 11 non-heterocystous cyanobacteria isolated from saline soil of Baramati region of Maharashtra. These isolates were evaluated for production of Indole-3-acetic acid in the presence of L-tryptophan. Out of 11 isolates, 9 isolates were able to produce IAA. Productivity of IAA was detected spectrophotometrically. IAA production of isolated cyanobacteria was evaluated in different pH, light photoperiods and various concentration of L-tryptophan, salt and nutrients. During 3 week incubation period TCC-1, TCC-4 and TCC-5 were producing higher amount of IAA (10.65µg/ml). TCC-1 and TCC-4 were found to produce higher amount (10.65µg/ml) of IAA in presence of 500,750 & 1000µg/ml of L-tryptophan concentrations. At pH 8, TCC-1 isolate was producing high amount (9.97µg/ml) of IAA. In the presence of light (24:00, L: D) conditions TCC-4 produces higher amount (9.42µg/ml) of IAA. In the presence of 200 mM and 400mM concentration of NaCl TCC-1, TCC-3 and TCC-4 produces IAA in higher amount respectively. Isolate TCC-5 was produces higher amount of IAA in presence and in absence of NaNO3. TCC-1, TCC-4 and TCC-5 cyanobacterial isolates were producing higher amount of IAA in optimized conditions. Keywords: Oxygenic photosynthetic prokaryotes, Non-heterocystous cyanobacteria, Indole-3-acetic acid (IAA), L-tryptophan, saline soil.
Authors and Affiliations
Bhosale Amarsinh, Puranik Pravin, Pawar Sunil
Keywords
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