Screening potential genotoxic effect of aquatic plant extracts using the mussel micronucleus test
Journal Title: Journal of Coastal Life Medicine - Year 2016, Vol 4, Issue 1
Abstract
Objective: To assess the genotoxic potential of selected aquatic macrophytes: Ceratophyllum demersum L. (hornwort, family Ceratophyllaceae), Typha angustifolia L. (narrowleaf cattail, family Typhaceae), Stratiotes aloides L. (water soldier, family Butomaceae), and Oenanthe aquatica (L.) Poir. (water dropwort, family Umbelliferae). Methods: For genotoxicity assessment, the mussel micronucleus test was applied. Micronucleus frequency was determined from the haemolymph of Unio pictorum L. (painter’s mussel). In parallel, total and hydrolisable tannin contents were determined. Results: All plant extracts elucidated significant mutagenic effect. Significant correlation was determined between tannin content and mutagenic capacity. Conclusions: The significant correlation between genotoxicity as expressed by micronucleus frequency and tannin content (both total and hydrolisable tannins) indicate that tannin is amongst the main compounds being responsible for the genotoxic potential. It might be suggested that genotoxic capacity of these plants elucidate a real ecological effect in the ecosystem.
Sri Lankan black tea (Camellia sinensis L.) inhibits the methylglyoxal mediated protein glycation and potentiates its reversing activity in vitro
Objective: To evaluate inhibitory activity of methylglyoxal (MGO) mediated protein glycation and ability to potentiate its reversing activity and range of antioxidant properties of Sri Lankan low grown orthodox orange pe...
Occurrence of Cryptosporidium oocysts in edible frogs ( Rana species) sold for human consumption in Hanwa frog market, Zaria, Kaduna State, Nigeria
Objective: To determine the occurrence of Cryptosporidium oocysts in edible frogs (Rana spp.) sold at the Hanwa frog market, Zaria, Kaduna State, Nigeria. Methods: A total of 117 frogs (Rana spp.) were randomly collected...
Analgesic, anti-inflmmatory and antipyretic activities of methanolic leaf extract of Maerua crassifolia
Objective: To investigate the the analgesic, anti-inflammatory and antipyretic activities of the methanolic leaf extract of Maerua crassifolia in mice and rats. Methods: Acetic acid-induced writhing and tail immersion me...
Effects of herbal plants (Zingiber officinale and Hibiscus sabdariffa) as dietary additives on serum biochemistry and some metabolites in Clarias gariepinus (Burchell, 1822)
Objective: To evaluate the effect of Zingiber officinale (ginger) and Hibiscus sabdariffa (roselle) as dietary supplements on serum biochemistry and some metabolites (urea and creatinine) in Clarias gariepinus (C. gariep...
GC-MS and NMR analysis of the bioactive compounds from the crude extracts of Waltheria indica and the histopathological changes induced in albino rats challenged with Naja nigricollis venom
Objective: To develop a characteristic fingerprint of the different chemical compounds of plant by gas chromatography-mass spectrometer (GC-MS) and nuclear magnetic resonance (NMR) analysis and assess the effect of the c...