Separation，identification and spore germination conditions of the pathogen causing shoot blight on blueberry （Vaccinium spp.）
Journal Title: 广东农业科学 - Year 2018, Vol 45, Issue 4
Abstract
"In 2016，some individuals of blueberry in Zhejiang plant base were dead in the seedling stage. In this study，to identify the pathogenic bacteria，some methods including tissue separation，single spore purification， Koch's postulate，conventional morphological and molecular biology were used. The factors affecting spore germination were also explored，including temperature，pH value，illumination condition，medium component and so on. Results showed that the dead plants were caused by shoot blight，which was induced by ZA1. The colony of pathogen ZA1 was round and white，and the conidium was spindle-shaped，divided into five cells by four true septums，and the intermediate cell was brown，the cells at both ends were colorless. The pathogen ZA1 was identified as Pestalotiopsis clavispora by morphological observation and sequence analysis of rDNA-ITS. Meanwhile，the optimum temperature for spore germination was 30℃，otherwise，lower temperature（ below 20℃） affected germination rate significantly. The adaptability of strain ZA1 spores was very broad，whose pH value could change among pH 3-12. And both light and complex carbon sources were beneficial for spore germination."
Authors and Affiliations
Mei LU, Wei-dong GUO
Keywords
Related Articles
- EP ID EP598439
- DOI 10.16768/j.issn.1004-874X.2018.04.017
- Views 72
- Downloads 0
Effects of different soil tillage practices on biological characteristics and grain yield of rice in double cropping rice field
Soil tillage practices are the key factor affecting soil physical properties in paddy field and grain yield of rice. Therefore，the variation of plant biological characteristics and grain yield of early rice and late ric...
Effect of deficient nutrients on growth and production of Prorocentrum lima
"The data reported here focus on the response of a tropical strain of the dinoflagellate（ Prorocentrum lima），isolated from South China Sea（ Sanya），to nutrient stress induced by various levels of nitrogen（ N） and...
Analysis of nitrate and nutrition of flowering Chinese cabbage in summer and winter in Guangzhou city
"In order to investigate nitrate content and nutrition of flowering Chinese cabbage in Guangzhou city， in summer and winter，30 varieties of flowering Chinese cabbage collected from four vegetable markets tested and ana...
Effects of cultivation methods on yield and quality of tomato…
"To understand the effect of different cultivation methods on yield and quality of tomato，a pot experiment was conducted with four tomato cultivars，Xinxing101 ，Jinbaolai ，kaidi Kaidi and Xiariyangguang， and three cu...
Analysis of volatile components of the peel of four different pummelos cultivars from Meizhou by GC-MS
In the present study，the volatile components of essential oils of Jin pomelo，Shatian pomelo， Aolan pomelo，and Mi pomelo from Meizhou city，Guangdong province were analyzed by gas chromatographymass spectrometry（ GC-...