Serological Detection of Equine Herpes Virus (EHV) Type 1 and 4 in Sudan
Journal Title: Microbiology Research Journal International - Year 2016, Vol 14, Issue 6
Abstract
Aims: This study was designed to investigate the presence of EHV 1 & 4 in blood serum of horses and donkeys in different localities in Sudan. Study Design: A total of 208 blood serum samples were collected from horse and donkeys from different localities in Sudan including; Khartoum, Atbara, Wadmadani and Nyala. With the exception of some horses (cross or foreign breeds), all animals examined were of Indigenous breed’s, different ages, and apparently healthy; clinical signs to equine herpes or any disease were not detected. Place and Duration of Study: The study was undertaken in the Virology lab, Veterinary Research Institute, Ministry of Animal Resources, Fisheries and Range Lands, Khartoum during December 2012 to February 2013. Methodology: Blood serum samples obtained from horse and donkeys were investigated in different localities in Sudan using an indirect ELISA (SVANOVIR® EHV1/EHV4-Ab). Results: EHV 1 was detected in 1.6% of the horse samples from Khartoum, Nyala and Wadmadani, and in 7.3% of the donkeys’ sample from Wadmadani and Nyala; while EHV 4 was detected (in all localities) in 58.7%, and 58.5% of the samples collected from horses and donkeys respectively. Mixed infection with both types (1 and 4) was recorded in horse samples from Khartoum and Nyala and in donkeys sample from Wadmadani and Nyala. Statistically, there is an association between infection and location (P –value < 0.001) Conclusion: Equine herpes virus 1 and 4 was detected for the first time in horses and donkeys in Sudan. High seroprevalence of EHV type 4 (58.7%, in horse and 58.5% in donkey’s serum samples) was recorded, compared to 1.6% and 7.3% of type 1 for horses and donkeys respectively. Mixed infection with both types 1 and 4 was recorded in horse samples from Khartoum and Nyala and in donkey’s sample from Wadmadani and Nyala. Further studies for virus detection such as PCR should be conducted to enables rapid identification of specific virus causing disease EHV I or EHV 4.
Authors and Affiliations
H. A. Wegdan, K. S. Intisar, M. M. Shaza, O. A. Algezoli, A. Ballal, M. E. Sahar, A. M. Baraa, H. S. Manal, E. A. Muna, K. M. Taha, E. M. Nada, Y. H. Ali
Keywords
Related Articles
- EP ID EP350920
- DOI 10.9734/BMRJ/2016/25803
- Views 107
- Downloads 0
Analysis of Amino Acid 530 and 549 of Hemagglutin Gene in Two Canine Distemper Virus Strains
Canine distemper virus (CDV) is prevalent among domestic dogs and causes disease in various types of carnivores worldwide. In the present study, the genotype of two CDV strains, namely, ZJJ-SD and ZJJ-LN, were investigat...
Comparison of Molecular Methods of Microbial Serotyping
Molecular serotyping methods have advantages and disadvantages in terms of different parameters. Since different methods depend on different parameters there is a possibility that one serotype detected by one method mayb...
Differentiation in a Single-Tube PCR between Leishmania mexicana and Leishmania braziliensis in Clinical Samples
Aim: To assess the usefulness of a single-tube PCR end point for differentiating between Leishmania and Viannia subgenera in clinical samples of cutaneous leishmaniasis. Methodology: Impression smears of 65 patients from...
Sero Prevalence of Equine Infectious Anemia (EIA) Virus in Selected Regions in Sudan
Equine infectious anaemia (EIA) is an acute to chronic lentivirus disease affecting members of equidae. In this study, the prevalence of EIA virus antibodies was investigated in 358 sera samples collected randomly from a...
Analysis of Influenza A/Beijing/353/89 Virus Matrix Gene Sequence and Identification of Replaceable Amino Acids as Natural Mutations in M2 Protein
The influenza virus A/Beijing/353/89 matrix (M), M1 and M2 genes were cloned and sequenced. The sequence identity rates (based on published data) of the first 34 influenza A viruses with M genes most phylogenetically rel...