Seroprevalence of Bluetongue Disease in the Cattle in Siirt Province, Turkey
Journal Title: IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS) - Year 2018, Vol 11, Issue 12
Abstract
Bluetongue is a viral disease of domestic and wild ruminants transmitted via the biting flies. The agent is in the Orbivirus genus of the Reoviridae family and has 24 known serotypes. The disease is most common during the summer months and is more severe during humid and rainy times. The aim of this study is to determine the presence of bluetongue infection serologically in cattle in Siirt province, Turkey, and to get information about its prevalence in the region. The material of the study consisted of a total of 279 cattle reared in different localities of Siirt. For laboratory analysis, 5 ml blood samples were taken from jugular vein of cattle and put into non-anticoagulant tubes. The samples were centrifuged at 3000 rpm for 10 minutes and serum was transferred into Eppendorf tubes and stored at -20 ºC until enzyme-linked immunosorbent assay (ELISA) analysis was performed. Serum samples were analyzed by an ELISA device (Multiskan GO, Thermo Scientific) using the commercial test kit (Bluetongue Virus VP7 Ab Test Kit, IDEXX). The results were evaluated as positive and negative. Of the 279 specimens analyzed, 150 (53.76%) were found to be seropositive and 129 (46.24%) were seronegative. As a result, in addition to measures for quarantine and vector control in the fight against the disease, it was concluded that vaccines specific to virus serotypes should be used and comprehensive and planned studies should be carried out in the region and across the country.
Authors and Affiliations
Ozgur Yasar Celik, Tekin Sahin
Keywords
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- EP ID EP421954
- DOI 10.9790/2380-1112013941.
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