Significance of Salivary Glutathione-S-transferase and Glutathione Reductase Enzymes in Oral Cancer
Journal Title: GUJARAT CANCER SOCIETY RESEARCH JOURNAL - Year 2016, Vol 18, Issue 2
Abstract
Oral cancer is a major health burden in India. Therefore, it is important and necessary to apply the vast knowledge that has been obtained regarding salivary biomarkers in oral cancer in the clinical area. Therefore, the aim of the study was to analyse salivary enzyme activity of glutathione-S-transferase (GST) and glutathione reductase (GR) as well as polymorphism in GSTM1 and GSTT1 genes from healthy individuals and oral cancer patients. Clinically diagnosed 57 oral cancer patients and 38 controls were enrolled for the study. Saliva samples were collected from the subjects. GST and GR enzyme activities were analyzed by spectrophotometric methods. PCR method was performed to study GSTM1 and GSTT1 polymorphisms. Statistical analysis was carried out by using SPSS software (Version 15). Mean salivary GR activity was significantly higher in oral cancer patients as compared to controls. Mean salivary GST activity was significantly lower in healthy individuals with tobacco habits as compared to healthy individuals without habit of tobacco. Mean salivary GST and GR activities were associated with clinicopathological parameters including tumor site, tumor differentiation and stage of the disease. In pilot study, GSTM1 null genotypes were higher (41.6%) in oral cancer patients as compared to controls (36.8%), whereas GSTT1 null genotypes were higher in controls (26.3%) as compared to patients (16.7%). Present study suggested that salivary analysis of antioxidant is simple and non-invasive technique which may be helpful in screening and as early diagnostic marker for oral cancer patients.
Authors and Affiliations
Dhara Bhimani, Kinjal Patel, Jayendrakumar Patel, Prabhudas Patel
Keywords
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- EP ID EP197216
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