SIMULTANEOUS DETERMINATION OF ANAESTHETIC AND ANTIBOTIC IN FLUID BY ELECTOCHEMILUMINESCENCE COUPLING CAPILLARY ELECTROPHORESIS
Journal Title: WORLD JOURNAL PHARMACY AND PHARMACEUTICAL SCIENCE - Year 2018, Vol 7, Issue 4
Abstract
The coupling of electrochemiluminescence (ECL) with capillary electrophoresis (CE) was developed for the simultaneous determination of lidocaine and lomefloxacin in human urine. Under the optimized conditions: ECL detection at 1.15V, 20mM phosphate buffer at pH 6.7, 5mM Ru(bpy)3 2+ and 60mM phosphate buffer at pH 7.6 in the detection reservoir, detection limits of 0.02ug mL-1 for lidocaine and 0.06ug mL-1 for lomefloxacin were obtained. Relative standard derivations of the ECL intensity and the migration time were 3.5 and 1.1% for 6ug mL-1 lidocaine, 3.2% and 3.7% and 1.2% for 6ug mL-1 lomefloxacin, respectively. Developed method was successfully applied to determine the amounts of lidocaine and lomefloxacin in human urine. A baseline separation for lodocaine, proline and lomefloxacin was achieved within 10 mins.
Authors and Affiliations
Su Ming
Keywords
Related Articles
- EP ID EP622523
- DOI -
- Views 149
- Downloads 0
ORAL SUSTAINED DELIVERY OF GASTROPROKINETIC DRUG MOSAPRIDE CITRATE FROM FLOATING MATRIX TABLETS-FORMULATION AND IN VITRO CHARACTERIZATION
Floating drug delivery systems can significantly prolong the gastric residence time of drug providing continuous input and greater therapeutic efficacy of the drug. The aim of the present study was to formulate effervesc...
MECHANISMS OF ACTION OF SOME ANTIDEPRESSANTS AND THEIR EFFICACY IN THE TREATMENT OF DEPRESSION
Depression is a moody condition linked with imbalance of neurotransmitter. In recent time depression is becoming one of the most prevalent public health problems. Abusive drugs, certain medication and life events such as...
AN OVERVIEW OF POLYAMIDOAMINE DENDRIMERS: STRUCTURE, PHYSICAL PROPERTIES, AND BIOMEDICAL APPLICATIONS
Polyamidoamine (PAMAM) dendrimers are the primary complete dendrimer family to be orchestrated, described and marketed. Dendrimers are hyper branched, monodispersed macromolecules with multivalent useful end bunches. Den...
DEVELOPMENT AND VALIDATION OF STABILITY-INDICATING RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF ROSUVASTATIN AND GLIBENCLAMIDE
A convenient and rapid reverse phase-HPLC (RP-HPLC) method has been developed and validated for simultaneous estimation of rosuvastatin (RSV) and glibenclamide (GBC) according to international conference on harmonization...
STUDY ON ALLERGY TRIGGERING FACTORS IN UNIVERSITY GOING STUDENTS IN KARACHI, PAKISTAN: A CROSS-SECTIONAL STUDY.
Objective: To study prevalence and pattern of allergy symptoms among young males and females Pharm D and medical students. Method: A prospective cross sectional study was designed based on validated survey questionnaire....