Special Stains to Disclose Barr Bodies in Buccal Scrape for Gender Determination in Forensic Science
Journal Title: International Journal of Contemporary Medical Research - Year 2017, Vol 4, Issue 3
Abstract
Introduction: Sex determination holds a valuable significance in these circumstances to clear medico-legal problems. In some instances the sample available might be very minute, in the form of only few cells which can be used to disclose the gender and identity of a person. One of the simplest methods of sex determination is visualizing Barr bodies from cytosmears of buccal mucosa using special stains. Study aimed to assess the easiest staining method for quick detection of Barr bodies to determine the sex of an individual using epithelial cells from buccal scrapes. Material and methods: A total of 50 individuals, 25 males and 25 females were considered for the study. Buccal scrapes and peripheral blood smears were obtained from each patient. Peripheral smears would be stained with Leishman’s stain and used as a standard whereas the cytosmears from buccal scrapes would be assessed for Barr bodies using routine hematoxylin and eosin stains and special stain alkaline methylene blue. Results: In females peripheral smears showed 100% positivity for Barr bodies whereas 88% cases were positive for special stain alkaline methyle blue and 80% cases showed positivity for hemotxylin and eosin stain. Conclusion: Alkaline methylene blue can be used as an easiest and quickest staining technique for sex identification.
Authors and Affiliations
Shruti Singh, Radhika M Bavle, Sreelatha S Hosthor, Reshma V, Sameer Vandrangi, Shiny Bopaiah
Keywords
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