Spectrophotometric Quantification of Anti-inflammatory Drugs by Application of Chromogenic Reagents
Journal Title: Turkish Journal of Pharmaceutical Sciences - Year 2019, Vol 16, Issue 4
Abstract
Objectives: Simple, specific, accurate, precise, sensitive, and cost effective spectrophotometric methods were developed and validated for quantification of the drugs lornoxicam (LOR) and mesalamine (MES) in pure form and in pharmaceutical formulations. Materials and Methods: A Shimadzu UV-1800 double-beam UV-Visible spectrophotometer having a spectral bandwidth of 0.1 nm with wavelength accuracy ±0.1 nm with a pair of 1 cm path length matched quartz cells was used to measure the absorbance of the resulting solution. Method I was used for the quantification of LOR, based on measurement of the absorbance of bluish green chromogen complex at 760 nm, which is formed by the reaction of LOR with ferric chloride and potassium ferricyanide (redox technique). Method II was used for the quantification of MES, based on measurement of the absorbance of yellow chromogen at 400 nm, which is formed by the condensation reaction of the primary amino group of MES with salicylaldehyde reagent (SA) (Schiff base formation). Results: Both methods obeyed Beer’s law in the concentration range of 0.5-4.5 μg/mL and 0.2-1.7 μg/mL with good correlation coefficients of 0.9974 and 0.998 for methods I and II, respectively. Conclusion: The developed method is simple, sensitive, and specific, which was validated statistically as per ICH guidelines, and can be used in the routine analysis of LOR and MES in pharmaceutical dosage forms.
Authors and Affiliations
Panikumar Durga ANUMOLU, Sunitha GURRALA, Archana GELLABOINA, Divya Gayathri MANGIPUDI, Sahitya MENKANA, Rajesh CHAKKA
Keywords
Related Articles
- EP ID EP670157
- DOI 10.4274/tjps.galenos.2018.07830
- Views 169
- Downloads 0
Screening Effects of Methanol Extracts of Diplotaxis tenuifolia and Reseda lutea on Enzymatic Antioxidant Defense Systems and Aldose Reductase Activity
Objectives: The aim of the study was to investigate the effects of methanol extracts from the flowers and leaves of Diplotaxis tenuifolia and Reseda lutea on the activity of AR, CAT, GST, and GPx. Materials and Methods:...
Carbon Quantum Dots: Synthesis, Characterization and Biomedical Applications
CQD are small carbon nanoparticles smallerl than 10 nm comprising distinctive properties, which have become an obligatory tool for traceable targeted delivery, biomedical research, and different therapy applications. The...
A Validated Reverse Phase-Ultra-Performance Liquid Chromatography Method for the Determination of Gemifloxacin Mesylate in Bulk and its Pharmaceutical Preparation
Objectives: Gemifloxacin Mesylate is a fourth generation fluoroquinolone antibacterial agent. A simple, accurate, and precise reversed phase (RP)- ultra performance liquid chromatography (UPLC) method was developed and v...
Flavonoids Isolated from Vitex grandifolia, an Underutilized Vegetable, Exert Monoamine A & B Inhibitory and Anti-inflammatory Effects and Their Structure-activity Relationship
Objectives: Vitex grandifolia belongs to family Lamiaceae; it consists of flowering plants and it is also called the mint family. The Yoruba people of southwest Nigeria called it “Oriri” or “Efo oriri”. This plant is cla...
Real-Time Cell Analysis of the Cytotoxicity of Origanum acutidens Essential Oil on HT-29 and HeLa Cell Lines
Objectives: The aim of this study was to investigate the cytotoxic and radical scavenging effects of the essential oil from Origanum acutidens (Hand-Mazz.) Ietswaart and to determine its chemical composition. Materials...