STABILITY-INDICATING REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR SIMULTANEOUS ESTIMATION OF METHYLCOBALAMIN, ALPHA-LIPOIC ACID, PYRIDOXINE HCL, AND FOLIC ACID IN BULK AND COMBINED DOSAGE FORM
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2018, Vol 11, Issue 12
Abstract
Objectives: The purpose of the research is to develop a simple, precise, economical, accurate, reproducible, and sensitive method for the estimation of methylcobalamin, alpha-lipoic acid, pyridoxine hydrochloride, and folic acid drug product by reversed-phase high-performance liquid chromatography (RP-HPLC) method.Methods: New analytical method was developed for the estimation of methylcobalamin, alpha-lipoic acid, pyridoxine hydrochloride, and folic acid in drug product by RP-HPLC. The chromatographic separation was achieved on the Inertsil C18, 250 mm × 4.6 mm, 5 μm at ambient temperature. The separation achieved employing a mobile phase consists of buffer (added 5.05 g hexane-1-sulfonic acid is dissolved into 1000 mL of distilled water):acetonitrile in the ratio of 10:90% v/v. The flow rate was 1 mL/min and UV-visible spectrophotometer at 285 nm. The average retention time for methylcobalamin, alpha-lipoic acid, pyridoxine hydrochloride, and folic acid was found to be 3.5, 6.7, 8.5, and 9.3, respectively.Results: The developed method was validated as per ICH guidelines. All validation parameters were within the acceptable ranges. The assay methods were found to be linear from 0 to 2130 μg/mL for methylcobalamin, 0 to 142.5 μg/mL for alpha-lipoic acid, 0–4.54 μg/mL for pyridoxine hydrochloride, and 0–2 μg/mL for folic acid. The correlation coefficient was 0.999 for all drugs, respectively. The mean percentage values for the developed method were found to be within the range of 98–100.6%. The developed method was also found to be robust.Conclusion: It is concluded that developed method was accurate, precise, linear, reproducible, robust, and sensitive.
Authors and Affiliations
Padmaja V, Prasanthi M, Mayuri P
Keywords
Related Articles
- EP ID EP605213
- DOI 10.22159/ajpcr.2018.v11i12.26715
- Views 140
- Downloads 0
INVESTIGATION OF EPIDEMIOLOGY AND ETIOLOGY OF LIVER DISEASES AND CHARACTERIZATION OF ITS ASSOCIATION WITH VARIOUS FACTORS
 Objective: The population of Gujarat is at higher risk of facing non-alcoholic liver diseases therefore it becomes important to examine etiology andepidemiology of liver diseases and to characterize its association to...
PHARMACODYNAMICS EFFECT OF METHYLPREDNISOLONE TABLETS ON THE SERUM CONCENTRATION OF ANNEXIN A1: IN VIVO COMPARATIVE STUDY BETWEEN GENERIC AND INNOVATOR DRUG
Objective: The aims of this study were to investigate the comparative pharmacodynamics effect of methylprednisolone (MP) innovator, MP branded generic, and MP generic products to the serum concentration of annexin A1 (An...
A DEEP INSIGHT ON DIABETIC NEUROPATHY: THE SILENT COMPLICATION OF DIABETES, WITH INPUTS ON ITS CAUSES, DIAGNOSIS, PATHWAYS, AND TREATMENTS
Background: The incidence of diabetic neuropathy (DNP) is a prominent complication for people suffering from diabetes. DNP is a common complication in patients suffering from diabetes, and it is affecting approximately m...
IN VITRO CYTOTOXIC ANTICANCER POTENTIAL OF BIOACTIVE FRACTION ISOLATED FROM INDONESIAN TIDAL SPONGE CALTHROPELLA SP.
Objective: This study was taken to examine the cytotoxicity of the bioactive fraction isolated from marine sponge Calthropella sp. as a preliminary anticancer assay and identify its bioactive compounds.Methods: The cytot...
A CASE REPORT OF ZIDOVUDINE-INDUCED THUMBNAILS HYPERPIGMENTATION IN AN HIV POSITIVE PATIENT WITH SECONDARY HERPES ZOSTER OPHTHALMICUS INFECTION
Zidovudine is one of the first-line antiretroviral therapy regimen drug used to treat human immunodeficiency virus infected patients. Nail pigmentation associated with zidovudine therapy is seen in adults and children’s,...