Studies on bioactivity and secondary metabolites of crude extracts of Bidens pilosa L. (Asteraceae): A medicinal plant used in the Transkei region of South Africa
Journal Title: Pakistan Journal of Pharmaceutical Sciences - Year 2016, Vol 29, Issue 3
Abstract
Whole plant-parts of Bidens pilosa were powdered and extracted in concentrated hexane, acetone, ethanol, methanol and water. The extracts were tested for antimicrobial activity against Escherichia coli (25922), Bacillus subtilis (ATCC 6051), Enterococcus faecalis (51299) Staphylococcus aureus (ATCC 29213) and Pseudomonas aeruginosa (ATCC127853), using standard microbiological techniques. Active crude extracts were macerated in concentrated methanol and tested for secondary metabolites including tannins, saponins, alkaloids, cardiac glycosides, anthraquinones, steroids and flavonoids using standard phytochemical procedures. Hexane and methanol extracts demonstrated similar activity producing 8-17 mm and 11-18 mm inhibition zone-diameter ranges respectively. Further analysis for minimum inhibitory concentrations (MIC50) recorded 1.25-20mg/mL and 2.5-20mg/mL for hexane and methanol extracts respectively. The highest zones of inhibition diameters (22-36mm) and lowest MIC50 values (0.0002-0.0006mg/mL) were recorded for Gentamicin, the positive control. Minimum bactericidal concentration (MBC) ranges were between 10-80mg/mL and 0.001-0.005mg/mL for extracts and control antibiotic respectively. With the exception of anthraquinones, the plant crude extracts tested positive for all secondary metabolites analyzed. These results provide scientific basis for the use of B. pilosa in South African traditional medicine. The antibacterial activity reported herein may be attributed to one or more of the 6 secondary metabolites detected in the plant crude extracts.
Authors and Affiliations
Collise Njume , Bomkazi M Gqaza , Carina Rozani , Nomalungelo I Goduka
Keywords
Related Articles
- EP ID EP138955
- DOI -
- Views 91
- Downloads 0
Phytochemical, antimicrobial, insecticidal and brine shrimp lethality bioassay of the crude methanolic extract of Ajuga parviflora Benth
Methanolic extract of medicinal herb Ajuga parviflora Benth. was evaluated for phytochemical screening (the plant extract showed the presence of aromatic compounds, carbohydrates, glycosides, tannins, alkaloids, po...
Antihyperglycemic effect of Persea duthieion blood glucose levels and body weight in alloxan induced diabetic rabbits
The present study was designed to investigate the antihyperglycemic effect of Persea duthieion blood glucose concentration and body weight in alloxan induced diabetic hyperglycemic rabbits. The results illustrated...
Purification and partial characterization of a Fructose-binding lectin from the leaves of Euphorbia helioscopia
A lectin was purified from leaves of Euphorbia helioscopia, by a combination of ion-exchange and gel filtration chromatography. On ion exchange using a DEAE- cellulose column in 0.2 M phosphate buffer, pH 7.2, the...
Distribution and clinical characteristics of pathogenic bacteria causing catheter-related bloodstream infections
This paper on analysis pathogenic bacterial distribution of central veins Catheter-related Blood-Stream Infection (CRBS) and clinical features of different infection. Ninety-one patients with CRBSI were selected, t...
Comparative evaluation of the anti-diabetic activity of Pterocarpus marsupium Roxb. heartwood in alloxan induced diabetic rats using extracts obtained by optimized conventional and non conventional extraction methods
The aim of the present study was to assess the anti-diabetic activity of Pterocarpus marsupium Roxb. heartwood in alloxan induced diabetic rats using extracts obtained by optimized conventional and non conventional...