Study of bacterial microbiota in patients with acute exacerbation of bronchial asthma and chronic bronchitis
Journal Title: Indian Journal of Microbiology Research - Year 2018, Vol 5, Issue 3
Abstract
Introduction: Microbiota of respiratory tract may be responsible for acute exacerbation of bronchial asthma and chronic bronchitis. So the present study was carried out to find out specific bacterial etiological agents’ involvement in acute exacerbation of these diseases. Materials and Methods: Sputum samples from patients of bronchial asthma, chronic bronchitis during acute exacerbation and endotracheal aspirates from control cases were processed to isolate the microbiota of respiratory tract using quantitative methods after liquefying and diluting the samples. Significant and insignificant bacterial counts from the samples were determined. Grading of pus cells and bacterial morphology was seen by Gram's staining. Results: Pus cell grading of 1+ to 3+ were seen in the both cases of bronchial asthma and chronic bronchitis whereas pus cells were not found in control cases. Percentage of microorganisms by Gram's staining in asthmatics, chronic bronchitis, and controls cases were 93.33%, 86.67%, 76.67% respectively. Among the pathogenic bacteria, percentage of significant count of Staphylococcus aureus and Klebsiella pneumonia were found to be high in chronic bronchitis cases when compared these pathogens in asthmatics. In bronchial asthma cases, the percentage of significant count of Streptococcus pyogenes, Proteus mirablis and Pseudomonas aeruginosa were high as compared to chronic bronchitis cases. In chronic bronchitis patients and asthmatics, significant count of non-pathogens were 16.67% and 13.33% respectively. Conclusion: The quantitative cultures showing significant numbers as 104/ml and 107 /ml of pathogenic and nonpathogenic bacteria respectively are found to be associated with acute exacerbation of bronchial asthma and chronic bronchitis.
Authors and Affiliations
Aruna Solanki, Arun More, Jyoti Tomar
Keywords
Related Articles
- EP ID EP472195
- DOI 10.18231/2394-5478.2018.0086
- Views 96
- Downloads 0
Screening for Methicillin Resistant Staphylococcus aureus (MRSA) colonization in health care workers, working in critical care areas at a tertiary care hospital, Karnataka, India
Introduction: Rise of MRSA infection in hospitals is a matter of concern. MRSA infection in hospitals is acquired either by MRSA colonizers i.e. health care worker or fomites which are heavily contaminated with MRSA. It...
Evaluation of urichrom –II for isolation of uropathogens in a tertiary care hospital
Urinary tract infections constitute a large share of human systemic infections and are one of the commonest bacterial infections that lead patients to seek medical care. Positive culture results are a prerequisite for ti...
Screening for group B streptococci in antenatal women: Isolation and its antibiogram
Group B streptococci(GBS) is one of those pathogens causing neonatal septicaemia and neonatal meningitis. Objective of this study is to screen antenatal women for Group B Streptococcus colonization and its antibiotic sen...
Spectrum of fungi causing onychomycosis in a tertiary care hospital in Bangalore
Introduction: Onychomycosis is a fungal infection of nails caused by dermatophytes, yeasts or non-dermatophyte moulds and represents about 30% of mycotic cutaneous infections. Onychomycosis manifests itself in various fo...
Identification and susceptibility pattern of Gram negative bacterial isolates of Catheter Associated Urinary Tract Infections (CAUTI) in a tertiary care institute
Introduction: Catheter associated urinary tract infection (CAUTI) is the most common health care associated infection worldwide. Multiple factors (type of catheter, insertion procedure, duration of catheter, handling of...