Study of CK5/6 in Benign and Malignant Breast Lesions
Journal Title: Journal of Medical Science And clinical Research - Year 2018, Vol 6, Issue 4
Abstract
Background: The presence or absence of a myoepithelial cell layer around carcinoma cells is the basis for dividing tumors into in situ and invasive types. Cytokeratin (CK) is a family of intracytoplasmic intermediate filament proteins present in almost all epithelial cells, which can be used to identify these myoepithelial cells and thus differentiate benign from malignant lesions. Moreover, If CK positivity is seen in malignant lesion, it indicates a basal type of carcinoma and have bad prognosis. Aims: The present study is designed to differentiate Benign Lesions and Malignant lesions and detect basal like carcinomas Material and Methods: This is a Cross – Sectional, Laboratory based comparative type of observational study in which immunohistochemical staining for cytokeratin 5/6 was applied on paraffin embedded sections of 100 breast lesions using avidin biotin peroxidase technique. The distribution and intensity of staining was recorded and graded semi quantitatively. Results: There were 50 cases of benign lesion, all of which were positive for CK5/6 except two cases of lactating adenoma. The malignant lesions comprised eleven cases of ductal carcinoma in situ (DCIS) and 39 cases of infiltrating carcinoma, not otherwise specified, IDC (NOS). None of the DCIS cases showed a positive immunoreaction but 19 cases of IDC were positive for CK5/6. The staining reaction in the malignant lesions was significantly less than that of benign lesions. Conclusion: The majority of carcinoma arising in women with BRCA 1 mutation are ‘basal like. ’Due to high proliferation and rapid growth, they carry an adverse prognosis. CK 5/6 can be used to identify these basal like subgroup and thus by screening there family members with BRCA1, prophylactic measures can be taken at an early stage..
Authors and Affiliations
Dr Aditi Jain
Keywords
Related Articles
- EP ID EP493317
- DOI -
- Views 62
- Downloads 0
The effect of single dose of preoperative dexamethasone on postoperative nausea and vomiting after laparoscopic cholecystectomy: a randomized, double-blind, placebo-controlled study
Objective: To study the efficacy of single dose of preoperative dexamethasone in the prevention of postoperative nausea and vomiting in patients undergoing laparoscopic cholecystectomy. Materials and Methods: 110 patient...
Protective effect of Apple Cider Vinegar on Stress Induced Gastric Ulcer
A gastric ulcer is a break in the normal gastric mucosa integrity that extends through the muscularis mucosa into the sub-mucosa or deeper. This work aimed to study the effect of chronic administration of vinegar on gast...
Neonatal Ascites– Experience from a Tertiary Care Centre
Objective: Neonatal ascites is rare but difficult problem for Neonatologist. To evaluate clinical investigational profile of Neonatal ascites this study was conducted in a city of northern India. Methods: The Neonates wh...
A Comparative Study on the Effect of Tobacco Smoke and Tobbaco Salivary Extract on Serum Lipid Profile
Background: Which form of tobacco use has more adverse effects on lipid profile? Objective: To identify the effect of tobacco use as smoking or chewing is associated with greater adverse effects on various components of...
A Comparative Study on Evaluation of Chalk and Board Over Power Point Presentation (PPT) As Methods of Teaching Anatomy to First Year Medical Students in Skims Medical College, Bemina
Background: The Study of Anatomy for a medical student is the basic building block for studying other branches of Medical Science and traditionally it has been taught by chalk and talk method and cadaveric dissection. Th...