Study of Factors Influencing Somatic Embryogenesis in Rice (Oryza Sativa L.)
Journal Title: International journal of Advanced Biological and Biomedical Research - Year 2015, Vol 3, Issue 1
Abstract
Objective: n present study effect of various factors including sucrose, agar, PEG, AgNO3, activated charcoal on somatic embryo induction and its subsequent regeneration was investigated using varieties Dom siah and Nemat. Methods: 330 explant (Mature embryos) of each varieties were cultured, 33/plate (100 x 15 mm), on callus induction Murashige and Skoog (MS) medium (Murashige and Skoog 1962) supplemented with 30 g/L sucrose, 7g/L agar and 2 mg/L 2,4-D. Results: Significant difference was observed between two variety in callus induction. Somatic embryogenesis and the plant regeneration were influenced by the genotype as well as composition of the medium used. The callus induction rate and callus morphology were different depend on genotypes. High somatic embryogenesis was obtained in medium (MS medium containing 2 mg/L 2,4-D) supplemented with 3 mg/L AgNO3, 9 gr/L agar and 20 gr/L PEG, respectively. The high frequency plant regeneration was achieved from both varieties in medium supplemented with 9 and 11gr/L agar and 5 mg/L AgNO3.
Authors and Affiliations
Hamideh Ghobeishavi| Graduated of Master of Science in Plant Breeding, Faculty of Agriculture, University of Tabriz, Tabriz, Iran, Ebrahim Dorani Uliaie| Assistant Professor, Department of Plant Breeding and Biotechnology, Faculty of agriculture, University of Tabriz, Tabriz, Iran, Seyed Siamak Alavikia| Assistant Professor, Department of Plant Breeding and Biotechnology, Faculty of agriculture, University of Tabriz, Tabriz, Iran, Mostafa Valizadeh| Assistant Professor, Department of Plant Breeding and Biotechnology, Faculty of agriculture, University of Tabriz, Tabriz, Iran
Keywords
Related Articles
- EP ID EP13460
- DOI -
- Views 399
- Downloads 13
Serological Survey of Antibodies to Toxoplasma Gondii in Sheep in North-West of Iran
Objective: Sera from 186 sheep (93 rams and 93ewes) from Tabriz, North-West of Iran were evaluated for antibodies to Toxoplasma gondii using a commercial ELISA kit. Methods: The animals were divided into two age groups...
Effect of water and temperature on seed germination and emergence as a seed hydrothermal time model
The physiological process of germination depends on several environmental factors such as temperature, water potential, light, nutrients and smoke. Water and temperature are determinant factors for seed germination. Bo...
Antimicrobial ‌in Vitro and in Vivo Potential of Five Lichen Species on Fusarium Equiseti and Pectobacterium Carotovora Pv. Carotovora Causal Agents of Potato Rots
Potato is one of the main crops which is suffering from the great losses in storage and the most conventional method of its tuber rot is using hazardous chemicals. Using antimicrobial potential of lichens can be one of t...
The Role of Climate and climatic factors on crop zoning agroclimatic sunflower cultivation in Hamedan province
Needs of human life from the beginning been an important factor We have been threatened by population growth, food security and human well- Men of science and technology with a new plan Maintain and improve their food...
Disclosing the Effect of Climatic Factors on the Growth and Yield of Corn in Kermanshah Province
The goal of this research is the determining of planting date and providing agricultural calendar for corn in Kermanshah province using climatic elements of temperature and rain fall. so, at first, long term climatic st...