Study of Oil Degrading Bacteria Isolated From Oil Contaminated Sites
Journal Title: International Journal for Research in Applied Science and Engineering Technology (IJRASET) - Year 2015, Vol 3, Issue 2
Abstract
Hydrocarbon degrading microorganisms were isolated from ten oil polluted areas in and around Navi Mumbai. Bushnell and Hass broth was used as selective enrichment medium with engine oil as a sole carbon and energy source and 19 different isolates were obtained. The oil degradative capacity of these isolates was evaluated using Emulsification index and Gravimetric analysis. The oil emulsification index was found to be in the range of 25 to 54%. Amongst the isolates, culture 1 showed best emulsification index and hence was used for further study. Oil degradative capacity of this isolate was found to be 41%. GC-MS study showed the conversion of Heneicosane to hexadecane indicating that the oil is partially degraded by the organism. Biochemical tests and 16s RNA sequencing confirmed that the isolate is Bacillus cereus.
Authors and Affiliations
Arpita Gupte, Sheetal Sonawdekar
Keywords
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