Study of transgene expression in Triticum aestivum L. after Agrobacterium-mediated in planta transformation
Journal Title: Вісник Українського товариства генетиків і селекціонерів - Year 2018, Vol 16, Issue 2
Abstract
Aim. Detection of sequences of target transgenes nptII and bar in the genome of probable transformants of bread winter wheat Triticum aestivum L. cultivars Zymoiarka and Podolianka obtained as a result of Agrobacterium-mediated transformation in planta and determination of their expression level. Methods. Polymerase chain reaction (PCR) method was used independently and in combination with reverse transcription (RT-PCR), electrophoresis of DNA in agarose gel. Tolerance to the herbicide was evaluated in the physiological test. Results Through PCR analysis, the sequence of nptII transgene was detected in 30 samples of 145 analyzed, the frequency of transformation was 20.7 %. The sequence of the gene bar was observed in 85 experimental plants, and the frequency of transformation was 15.6 %. mRNAs of both transgenes were detected, indicating their transcriptional activity and stable expression. Conclusions PCR analysis allowed to detect nptII transgenic signal in 20.7 % of plants, while the presence of the bar gene was detected in 15.6 % of cases, indicating a higher efficiency of this genetic construct. The transcription is shown in all the specimens studied for both transgene. According to the results of the physiological test, 25 % of plants containing the gene bar showed resistance to the Basta® herbicide.Keywords: genetically modified organisms, transgenic plants, biotechnological cultures, bread winter wheat, genetic engineering.
Authors and Affiliations
N. A. Zhalii, M. O. Bannikova, L. H. Velikozhon, A. M. Taranenko, B. V. Morgun
Keywords
Related Articles
- EP ID EP613127
- DOI 10.7124/visnyk.utgis.16.2.1053
- Views 116
- Downloads 0
Genetic transformation of Populus nigra X P. deltoides (black poplar, clone Gradizka)
Aim. To carry out genetic transformation of poplar Populus nigra x P. deltoides clone Gradizka with the model gene construct pCB002 carrying selective gene of kanamycin resistance and marker gene of β-glucuronidase. Meth...
Molecular docking study of oligonucleotides with D-mannitol
Aim. Complex of yeast RNA and D-mannitol has a specific antiviral activity. This complex inhibits the neuraminidase and hemagglutinin activity of viruses such as influenza, parainfluenza and thus it blocks virus entry in...
Stem potential of the new human cell line 4BL
During generation of new cell line the researchers are primarily interested in such characteristics as morphology and immunophenotype, which allows attributing beloning of the cell to that or other type and accordingly o...
Богдан Павлович Мацелюх (до 85-річчя від дня народження)
Аналіз експресії ізоформ ITSN2 та TKS5 в пухлинах грудної залози людини
Мета. Незважаючи на великий прогрес у лікуванні, 15 % випадків раку молочної залози залишаються летальними. Попри багаторічні дослідження та великий досвід у лікуванні цього виду раку, однією з основних проблем в діагнос...