Survey of Silver Nanoparticles Antibacterial Activity Against Gram-Positive and Gram-Negative Bacteria in Vitro
Journal Title: The Journal of Tolooebehdasht - Year 2017, Vol 16, Issue 1
Abstract
Introduction: Increasing resistance to antimicrobial agents is typically one of the main problems of health care. Control the dispersion of bacteria in different environments is a challenge in this section that to the overcome of them is essential for health and economic. In this study, nano-silver antibacterial activity was studied against Gram-positive bacteria Staphylococcus aureus and Pseudomonas aeruginosa and Gram-negative bacterium E. coli and Bacillus cereus. Methods: Anti-bacterial properties of silver nanoparticles against bacteria were assessed using dilution method. Bacteriological tests were performed using the initial concentration of (1 -1.5× 10-5) CFU / ml of each type of bacteria. The minimum inhibitory concentration (MIC) of growth and minimum bactericidal concentration (MBC) was determined for each bacteria in the agar medium. Since the death of bacteria was studied using a double-MIC concentrations in duration of zero to 540 min and was determined the death time of each bacteria. Results:The results showed that MIC for bacteria of E. coli, Bacillus cereus, Staphylococcus aureus and Pseudomonas aeruginosa was at a concentration of 1700, 1600, 1500, and 1600 microgram per liter of silver nanoparticles, respectively. Also, the lowest death time was obtained 4h, for Bacillus cereus bacteria. Conclusion: Based on the results of this study, bacteria E. coli showed the lowest sensitivity and Staphylococcus aureus the greatest sensitivity to silver nanoparticles.
Authors and Affiliations
Mohammad Hossien Salmani, Mahbobeh Mirhosseini, Mohsen Moshtagi laregani, Khadijeh Akrami
Keywords
Related Articles
- EP ID EP261051
- DOI -
- Views 145
- Downloads 0
Adsorption of Phosphorous on Natural and H2O2 Modified Pumice
Introduction: Phosphate is known to be the main limiting elements in the reuse of wastewater effluents and then it is the key element to outbreak of eutrophication. Therefore the objective of this work was to investigate...
Gender Identity Disorder and Social Factors Affecting it Among Transsexualists, Who Referred to the Social Welfare Organization in 2013-2015
Introduction: Gender identity disorder refers to the most extreme level of sexual boredom and is defined as a feeling of discomfort with one’s own sexual anatomy and the desire to get rid of one’s genital organs and livi...
Prevalence of Listeria Monocytogenes in Traditional ice Cream, Yazd, IRAN (2015) and Compared to Other Studies in Different Parts of Iran
Introduction:The bacterium causes listeriosis with severe clinical consequences such as meningitis, septicemia and abortion. There are a minimum data on dairy products contamination of Listeria in Iran. Thus, the objecti...
Study of Prevalence &Risk Factors of Congenital Heart Defect (Review Article)
Introduction: Congenital heart defects are known as the state that comes from birth and influences on structure and function of baby's heart, The different types of defects can range from mild (e.g., a small hole between...
Using of Moisture Term in the Dynamic Modeling in Treatment Process of Hexane-Contaminated Air
Introduction: An effective method for biological treatment of contaminated air to organic volatilization compounds is the biofiltration method, which the pollutant substance is transmitted by air passing through the gas...