Survey of single strand conformation polymorphism of kappa-casein gene in Alpine and Saanen goats in Iran
Journal Title: Animal Science Papers and Reports - Year 2013, Vol 31, Issue 2
Abstract
To study the genetic polymorphism of Îș-casein (CSN3) gene as candidate gene for marker-assisted selection in different domestic livestock species, blood samples were randomly withdrawn from 34 Alpine and 42 Saanen goats. DNA was extracted from blood samples and a 416 bp fragment from exon 4 of the CSN3 gene was amplified by polymerase chain reaction (PCR). PCR products were analysed using single strand conformation polymorphism (SSCP) method. Results indicated that there were two (K1 and K2) conformational patterns with frequencies 0.91 and 0.09 for Alpine and 0.76 and 0.24 for Saanen goats, respectively. A Chi-square test confirmed Hardy-Weinberg equilibrium for the gene in both breeds.
Authors and Affiliations
Rasoul Khatami Nejhad, Soheil Yousefi, Mojtaba Ahani Azari
Keywords
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