Susceptibility of Mycosphaerella fijiensis isolates to the selection agents Hygromycin B and Carbendazim Enviar comentarios Historial Guardadas Comunidad
Journal Title: Biotecnología Vegetal - Year 2006, Vol 6, Issue 4
Abstract
The study of Musa - Mycosphaerella fijiensis interactions supported by molecular tools such as the genetic transformation could contribute to elucidate new mechanisms related with the pathogenesis and through it to allow the development of new selective fungicides as well as the elaboration of more durable resistance strategies. This work had as objective, to determine the Minimum Inhibitory Concentration (MIC) of different antimicrobial substances commonly used as selection agents in the genetic transformation study of different species of Mycosphaerella (Hygromycin B and carbendazim). The agar dilution method was used and mycelial suspensions of nine of M. fijiensis isolated by several regions of Villa Clara and Ciego de Ávila was used as inoculums. Concentrations of these substances from 32.0 to 0.015 mg.l l -1 were rehearsed. It was possible to determine the MIC of both antimicrobials substances against the M.fijiensis isolated using this method. The MCI of the Hygromycin B for 100% of the isolated was less to 8 μg.ml l -1 and the rehearsed 90% of the isolated ones showed, for the carbendazim, MIC less or similar to 0.125 μg.ml l -1 . CCIBP-Pf-57 isolated did not achieve to be inhibited to the rehearsed concentrations of the Carbendazim. The results obtained in this work evidence the importance of knowing the MIC of the selective agents that are used in the transformation avoiding escapes in the selection.
Authors and Affiliations
Mileidy Cruz-Martín , Yelenys Alvarado-Capó, Mayra Acosta-Suárez , Michel Leiva-Mora1 , Berkis Roque , Minerva Lezcano
Keywords
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