Te Antibacterial Activity of Honey on Helicobacter Pylori
Journal Title: Sultan Qaboos University Medical Journal - Year 2006, Vol 6, Issue 2
Abstract
Objective: Tis project aimed to assess the antibacterial potential of various brands of honey sold in Muscat area on some isolates of H. pylori and to determine if there is any synergy between honey and amoxycillin or clarithromycin used in the treatment of H. pylori gastritis and duodenal ulcer. Methods: Eight samples of commercial honey were used in the experiment after they were checked for purity by sub-culturing on blood agar and incubating for 48 hours at 370c. Honey samples showing gross contamination were discarded. Purified culture isolates of H. pylori from our laboratory stock cultures were swabbed on chocolate plate using x 04 cfu/ml. One hundred microlitres (00µl) of various honey samples were placed on each plate which was subsequently incubated microaerophilically at 37ºc for 3 days. Te presence or absence of growth inhibition zones on each plate was noted and an average zone size of each honey was taken. Honey samples with high zone sizes were further diluted from :2-:8 to find the end-points of their growth inhibition concentrations and the experiment was repeated in triplicates. Te synergistic effect between honey, amoxycillin and clarithromycin was done in triplicates by placing honey at various distances between each antibiotic after swabbing chocolate agar with x 04 cfu/ml of H. pylori. Te plates were incubated as before. Results: All honey samples produced growth inhibition zones with H. pylori no at dilution of honey but had different zone sizes at :2–:8 dilutions. Black Forest honey had the highest antibacterial activity followed by Langnese honey. None of the honeys had a synergistic effect with either clarithromycin or amoxycillin. Conclusion: We conclude that, in vitro, some honey brands possess antibacterial activity against H. pylori and that no synergy or antagonism was observed between honey and clarithromycin or honey and amoxicillin using H. pylori as a test organism. Tough no synergy or antagonism was observed between honey, amoxicillin or clarithromycin, it has been suggested that the use of honey with triple therapy regimen may help shorten the time required to eliminate H. pylori from stomach lining of patients with gastritis or duodenal ulcer caused by H. pylori infection.
Authors and Affiliations
Basil C Nzeako| Department of Microbiology and Immunology, Sultan Qaboos University, College of Medicine and Health Sciences, P.O.Box 35, Al-Khod, Muscat 123, Sultanate of Oman, Faiza Al-Namaani| Department of Microbiology and Immunology, Sultan Qaboos University, College of Medicine and Health Sciences, P.O.Box 35, Al-Khod, Muscat 123, Sultanate of Oman
Keywords
Related Articles
- EP ID EP13694
- DOI -
- Views 391
- Downloads 18
Impact of Home-to-Centre Distance on Bone Marrow Transplantation Outcomes
Objectives: Haematopoietic stem cell transplantation (HSCT) in Oman started in 1994 at Sultan Qaboos University Hospital (SQUH), Muscat, Oman. Previous studies have suggested that longer driving time to the transplant ce...
Randomised Trial of Oral Misoprostol Versus Manual Vacuum Aspiration for the Treatment of Incomplete Abortion at a Nigerian Tertiary Hospital
Objectives: This study aimed to compare the efficacy of oral misoprostol with manual vacuum aspiration (MVA) in first trimester incomplete abortions. Methods: This randomised controlled trial study was conducted at the U...
Submyomatous Cornual Pregnancy Managed surgically after failed medical management
Cornual pregnancy constitutes an emergency while its diagnosis and management remain a challenge. Anatomical abnormalities in the uterus, such as fbroids in the cornual region, make the management even more difficult....
A Review of Obstetrical Outcomes and Complications in Pregnant Women after Bariatric Surgery
Bariatric surgery (BS) is a novel treatment for weight reduction with longer lasting health benefits. This review aimed to summarise the available evidence regarding the fetomaternal outcomes and the most common challeng...
Cardiac Markers in the Early Diagnosis and Management of Patients with Acute Coronary Syndrome
Chest pain is a non-specifc complaint and is the most frequent reason for patients seeking urgent medical attention. A small group of these patients will have acute coronary syndromes (ACS). Te current diagnostic and t...