Tea Polyphenols Synergistic Enhancement of Antibacterial Effects of NaF on the Cultured Streptococcus Mutans
Journal Title: Biomedical Journal of Scientific & Technical Research (BJSTR) - Year 2018, Vol 6, Issue 2
Abstract
It is reported that about 95% of the populations in the world have dental caries. Although NaF is an effective anticariogenic agent and F- is the strongest electronegative ion, the abuse of NaF is not only harmful to human beings (dental fluorosis, hepatotoxicity, nephrotoxicity etc.) but also an ecosystem destroyer. It is urgent to explore a better anticariogenic agent for prevention and therapy of dental caries. In this study, we have comparatively studied the antibacterial effects of four different kinds of tea polyphenols on the cultured cariogenic Streptococcus mutans UA159. The results obtained showed that tea polyphenols are more potent than NaF in inhibiting the growth of UA159 which is based on the concentrations (mM) required for 50% inhibition. An important finding is that black tea polyphenol is not only by itself to inhibit the growth of UA159 but also synergistically enhanced this inhibitory effect of NaF. Furthermore, tea polyphenols were also much more potent than NaF in inhibiting α-glucosidase activities of UA159. In conclusion, the antibacterial effects of tea polyphenols on the cultured anticariogenic Streptococcus mutans UA159. It is expected that tea polyphenols could be developed as promising anticariogenic agents in the future. Dental caries is the most common disease in the dentistry. It is estimated that 95% of populations in the world have this disease [1,2]. Thus, its medical cost is enormous greater than those on cancer, cardiovascular diseases, diabetes etc. Although the etiology of dental caries is explored, it is very difficult to eradicate. It is urgent to explore a better regimen for prevention and therapy of dental caries. Streptococcus mutans is recognized as a cause of dental caries. It can degrade sugar into glucose which is polymerized into glucan [3,4]. Dental plaque containing 70 % of S. mutans and glucan was performed on the enamel surface. S. mutans can secret organic acids, which destroyed enamel and eventually led to dental caries. The effective anticariogenic NaF not only possessed anti-bacterial effects but also altered enamel structure to synthesize aciduratic CaFPO4 [5-7]. However F- is the most electronegative ion, not only highly toxic to tissues and cells (fluorosis, hepatotoxicity and nephrotoxicity), but also destroying our ecosystems [8-11]. It is very important to develop a better anticariogenic agent to replace or reducing the consumption of NaF and its analogues. Tea polyphenols such as green tea polyphenol (EGCG) has been demonstrated to be a potent anti-cariogenic agent [12-15]. However, there are four kinds of teas commonly used (unfermented green tea, half-fermented oolong tea, complete fermented black tea and post fermented Puerh tea). In our laboratory, we have demonstrated that both black tea and puerh tea are the best to modulate obesity and diabetes, and cancer chemoprevention [16-22]. Because there are still no reports on the comparative potencies of the anticariogenic effects of the teas, thus, in this study, we attempted to explore tea polyphenols either alone or in combination with NaF on the growth, acid secretion, biofilm formation and α-glucosidase activities of the cultured S.mutaus UA159. We have demonstrated that the antibacterial effect of black tea polyphenols was not only the most potent antibacterial, but also exhibited synergistic inhibitory effect in combination with NaF on UA159.
Authors and Affiliations
Shoei Yn Lin Shiau, Chia Tze Kao
Keywords
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- EP ID EP585654
- DOI 10.26717/BJSTR.2018.06.001328
- Views 180
- Downloads 0
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