TESTICULAR GENE EXPRESSION PROFILING OF PHENYTOIN TREATED ALBINO RATS USING CDNA MICROARRAY
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2013, Vol 1, Issue 5
Abstract
To propose Testicular gene expression profiling of phenytoin treated albino rats using cDNA microarray analysis. Phenytoin is a commonly used antiepileptic. Phenytoin controls the abnormal brain activity observed in seizure by decreasing electrical conductance among brain cells by stabilizing the inactive state of voltage-gated sodium channels. Phenytoin may alter the secretion and functions of different hormones which may attribute to sexual dysfunction In order to evaluate the effects of phenytoin on the reproductive system of rats at molecular level in detail, cDNA microarray analysis was used in which about 44000 genes of test and control samples were analysed for differential regulation by comparing test with control samples. In this study, the albino rats were divided into two groups, control and test. The test group was given 120mgs/kg /body weight of phenytoin orally and equal amount of normal saline was given for the control group. After 45 days with the rat under deep anaesthesia, the testis were removed from the scrotum and stored in liquid nitrogen. The stored specimens of testis of control and tests group were subjected to cDNA microarray analysis and also small quantity of the tissue was subjected for Histopathological examination. This study showed the differential expression of genes in test group when compared with the control group. Out of 44000 genes analysed, 2273 were up regulated and 4430 genes were down regulated and further gene cluster analysis was performed to identify testis specific differentially regulated genes and the histopathological examination also revealed histopathological changes in the Test group.
Authors and Affiliations
Rajkumar R, Vathsala Venkatesan, Sriram Thanigai
Keywords
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