The activity of N-acetyl-β-glucosaminidase and its isoenzymes in the renal tissue, serum and urine of patients with renal cancer
Journal Title: Współczesna Onkologia - Year 2005, Vol 9, Issue 7
Abstract
Renal cancer makes up around 3-4% of human neoplasms. It may metastasise, infiltrate and colonise other tissues. Infiltration and metastasis may depend on changes in the oligosaccharide structures of glycoconjugates of the glycocalyx, basement membranes and extracellular matrix. Oligosaccharides are degraded by endo- and exoglycosidases. N-acetyl-β-glucosaminidase (NAG) produced mainly by the epithelial cells of proximal convoluted renal tubules, is the most active of all exoglycosidases. N-acetyl-β-hexosaminidase is a lysosomal exoglycosidase which hydrolyses β-glycosides of N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc) from glycoconjugates. In human tissues it exists as two major isoenzymes: a thermolabile NAG A and thermostabile NAG B. NAG is a glycoprotein composed of two subunits α and α. Isoenzyme A has composition αβ isoenzyme B ββ, respectively. Genes coding subunit α NAG are localised to chromosome 15, and those coding subunit β are localised to chromosome 5. The cancerous tissue revealed a significantly lower activity, of NAG and its isoenzyme A, and transitional tissue showed an intermediate activity in comparison to control renal tissue. A significant increase in NAG activity was observed in the serum and urine of renal cancer patients, in comparison to control subjects. In conclusion we propose determination of NAG in urine as a potential marker of renal cancer.
Authors and Affiliations
Małgorzata Borzym-Kluczyk, Barbara Darewicz, Sławomir Szajda, Marian Sulik, Krzysztof Zwierz
Colorectal cancer - adjuvant chemotherapy, and immunotherapy for advanced disease with cetuximab monotherapy, and cancer chemotherapeutic combination
Colorectal cancer appears to be a real challenge for modern oncology. The global morbidity and mortality represent 875 K new cases and 570 K deaths per annum, respectively. A human population affected by colorectal cance...
Activity of β-galactosidase and α-mannosidase in the serum of patients with colon adenocarcinoma
Exoglycosidases β-galactosidase (GAL) and α-mannosidase (MAN) take part in the synthesis of glycoconjugates in the endoplasmatic reticulum and Golgi apparatus, as well as their degradation in the lysosomes. An increase...
How 5-fluorouracil acts
5-fluorouracil (5-FU) has proved to be one of the most effective chemotherapeutics for colorectal cancer. It is also used as a drug against breast, head and neck, esophageal, gastric, and several other cancers. Intracell...
Receptory estrogenowe i progesteronowe w raku piersi– współczesny stan wiedzy
Obecność receptorów estrogenowych i progesteronowych w komórkach raka piersi stanowi bardzo silny i użyteczny czynnik predykcyjny. Ponieważ odsetek odpowiedzi na leczenie hormonalne jest ściśle związany z zawartością rec...
Nowe możliwości leczenia kwasem zoledronowym przerzutów do kości w przebiegu raka gruczołu krokowego, na tle zdobyczy badań molekularnych
Rak gruczołu krokowego jest nadal poważnym wyzwaniem. Na świecie stale obserwuje się ogromną liczbę zachorowań. Rak gruczołu krokowego jest głównie rozpoznawany w krajach wysoko uprzemysłowionych. Sposób odżywiania i try...