The Ameliorative Effects of Ethyl Pyruvate and Dimethyl Sulfoxide on Ischemic Tissue Injury in Experimental Carbon Monoxide Intoxication
Journal Title: Eurasian Journal of Emergency Medicine - Year 2020, Vol 19, Issue 1
Abstract
Aim: The purpose of this study was to investigate the protective effects of ethyl pyruvate (EP) and dimethyl sulfoxide (DMSO) on ischemic tissue in brain and cardiac and hepatic tissues in experimentally induced carbon monoxide (CO) intoxication. Material and Methods: Thirty-five mature female Sprague Dawley rats were randomized into five groups of seven animals each. Group 1 received no CO or treatment. Rats in groups 2, 3, 4 and 5 were made to inhale a high-concentration 5000 ppm CO gas mixture for 60 min at 4 L/min. CO levels were then measured from 1 mL tail vein blood. Group 2 received no therapeutic agent, group 3 received 6 mg/kg intraperitoneal (ip) DMSO, group 4 received 50 mg/kg ip EP, group 5 received 50 mg/kg EP and 6 mg/kg DMSO ip. All rats were sacrificed by decapitation. Brain, cardiac and hepatic tissues were removed and histopathological scores were compared. Results: Comparison of group 2 with group 3 and group 2 with group 5 revealed that DMSO alone and EP + DMSO exhibited a reducing effect on degree of cerebral neuronal alteration, degenerative neuron rates, and total cardiac injury score (p=0.005, p=0.002, p=0.004 and p=0.002, p=0.001, p=0.004 respectively). There was no histopathological difference between group 2 and group 4 suggesting EP alone had no therapeutic effect on histopathological injury. Conclusion: Based on our study findings, EP administered alone exhibited no protective effects on the organ injuries investigated, while DMSO exhibited reducing effects on degree of neuron alteration, rates of degenerative neurons, and total cardiac injury scores. An ameliorating effect on cardiac and hepatic injury was more prominent with combined treatment.
Authors and Affiliations
Sinan Paslı, Melih İmamoğlu, Mustafa Çiçek, Metin Yadigaroğlu, Aynur Şahin, Yunus Karaca, Esin Yuluğ, Özgür Tatlı
Keywords
Related Articles
- EP ID EP684351
- DOI 10.4274/eajem.galenos.2019.20438
- Views 176
- Downloads 0
Evaluation of Serum 25-Hydroxyvitamin Vitamin D, Vitamin B12, and Folate Levels in Patients with Benign Paroxysmal Positional Vertigo
Aim: This study aimed to compare 25-hydroxyvitamin D (25-OH Vit D), vitamin B12, and folic acid levels in patients presenting with vertigo diagnosed with benign paroxysmal positional vertigo (BPPV) and healthy volunteer...
Initial Versus Final Diagnosis in Patients Who Presented to the Emergency Department Without Trauma: A Prospective Cohort Study
Aim: In the emergency department (ED), some patients are discharged after their initial diagnosis and treatment, whereas others are hospitalized for treatment and/or further diagnostic examination. The ED physician usual...
Thirty-Day Mortality in Septic Shock Patients is Directly Associated with High Disease Severity Score but not with the Length of Stay in the Emergency Department
Aim: To determine the factors associated with mortality in septic shock patients who are transferred from the emergency department (ED) to the intensive care unit (ICU). Materials and Methods: We used the data of 206 pa...
Bougie-facilitated Intubation in Penetrating Neck Injury
A young male patient with a history of schizophrenia was admitted to the emergency department after suicide attempt. He slashed his neck with a broken glass and this resulted in a wound in zone II exposing laryngeal stru...
Acute Management of Spinal Cord Injury in Out-of-hospital and Emergency Department Settings
Spinal cord injury (SCI) is a devastating neurological disorder that is estimated to affect approximately 1000 patients each year in the Republic of Turkey. With this review, we aim to update the recent evidence related...