THE CHARACTERISTIC OF THE FUNCTIONING OF THE SELECTED COMPONENTS OF PLANT ANTIOXIDANT SYSTEM IN LIGULARIA GLAUCA (L.) HOFFM. AND L. SIBIRICA (CASS.) TISSUES UNDER THE DIFFERENT GROWING CONDITIONS
Journal Title: Naukovyi visnyk Chernivetskoho universytetu. Biolohiia (Biolohichni systemy) - Year 2014, Vol 6, Issue 2
Abstract
The quantitative content of the amounts of polyphenols, flavonoids and polyphenol oxidase activity of Ligularia glauca (L.) J. Hoffm. and L. sibirica (Cass.) explants was observed by analyzing the functioning of the antioxidant protection system of plants. The plants were grown in the presence and absence of the exogenous cysteine in the growth medium. The research subjects were L. glauca і L. sibirica explants which were grown under in vitro conditions in the presence of 60 mg/l cysteine in medium and without it. The Murasihe-Skoog medium was the base for the preparation of the culture mediums. The content of the amounts of polyphenols and flavonoids was determined spectrophotometrically in terms of routine and chlorogenic acid accordingly. The еlectrophoretic studies of the polyphenol oxidase isoforms were performed in native conditions in the 5% concentrating and 12% sharing polyacrylamide gels. The tris-glycine buffer рН 8,3 was used as the electrode buffer. The gels were incubated in 0,1% L-DOPA in the sodium phosphate buffer рН 7,0 for the identification of enzyme. It is shown that L. glauca and L. sibirica explants are able to accumulate large enough number of the polyphenolic compounds. However, their content is twice lower in L. glauca raw in the presence of in the growth medium. In L. sibirica explants the flavonoids content is more than twice predominated by such a different species. This difference increases significantly under their cultivating condition in the presence of exogenous cysteine. The polyphenol oxidase activity of explants depends also on the cultivating peculiarities. The minimum enzyme activity is set for L. glauca explants, which were grown with the absence of exogenous cysteine in the culture medium. The activity values for both explored types are different almost in 20 times. It is shown, that the isozyme spectrum of L. sibirica polyphenol oxidase consists of ten components, while L. glauca of six. It was discovered his dependence on the cultivating conditions. Thus, L. sibirica consistently shows nine components, while L. glauca electrophoretogram – four. The component with Rf becomes majeure colour under stressful growth conditions in L. sibirica and appears in L. glauca. Now we can talk about higher adaptive capacity of L. sibirica based on the qualitative and quantitative analysis of components of the enzymatic and nonenzymatic part of the antioxidant L. glauca and L. sibirica protection system.
Authors and Affiliations
А. Є. ШЕЛИФІСТ, М. Д. ДЗВІНЧУК
Keywords
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