The Characterization of Amylolytic Enzyme Present in Fermented Sweet Sap of Palmyrah
Journal Title: Journal of Applied Biology & Biotechnology - Year 2016, Vol 4, Issue 3
Abstract
Amylase enzyme obtained from 12h of naturally fermented sweet sap of palmyrah. The amylase showed zero order kinetics for 10min. The optimum temperature for the activities of enzyme as 65oC and pH was 7.0. When the enzyme was pre-incubated at 65oC and at pH 7.0, it lost 62% of its initial activity at 60min. This enzyme showed activity with starch, lactose, maltose, pectin and sucrose. Amylase activity was strongly inhibited by 2mM of Hg2+ but 2 mM of Ca2+, Ba2+, Mg2+, Mn2+, Na+, Zn2+, Mn2+ and Cu2+ stimulated the enzyme activity at 65 oC and at pH 7.0. The enzyme in the presence of 2mM Mn2+, Cu2+ and Na+ separately, retained 79, 86 and 68 % of its initial activity respectively at 60 min, 65oC and pH 7.0.
Authors and Affiliations
Keywords
Related Articles
- EP ID EP456311
- DOI 10.7324/JABB.2016.40304
- Views 88
- Downloads 0
Bioremediation of heavy metals from aquatic environment through microbial processes: A potential role for probiotics?
Heavy metals can cause disastrous effects on any living organisms. Probiotics have the ability to reduce metal toxicity. Bioremediation of polluted waters using these bacteria could be an alternative to conventional reme...
Chitosan and \ß-amino butyric acid up-regulates transcripts of resistance gene analog RGPM213 in pearl millet to infection by downy mildew pathogen
Plants are hosts to a diverse group of pathogens belonging to different kingdoms of life. In absence of active immune system, plants have evolved several layers of defense to combat individual pathogen strain and evolvin...
Screening and optimization of culture conditions of Nannochloropsis gaditana for omega 3 fatty acid production
Omega-3 fatty acids are essential fatty acids which are necessary for human health obtained only through diet. The three types of omega-3 fatty acids are involved in human physiology are alpha linolenic acid found in pla...
Klebsiella pneumoniae VRE36 as a PGPR isolated from Saccharum officinarum cultivar Co99004
Klebsiella species known to exhibit important PGP traits like solubilization of phosphate, phytohormone production and good germination potential. In present study, based on the Phosphate solubilization and IAA productio...
Isolation and molecular characterization of bacteria from the gut of Eisenia fetida for biodegradation of 4,4 DDT
There is a growing interest in the bio-application of microorganisms from the gut of earthworms on the bio-transformation of persistent and toxic pollutants during vermifiltration. Earthworms harbor beneficial microbes i...