The Cytotoxicity of Aflatoxin B1 in Human Lymphocytes
Journal Title: Sultan Qaboos University Medical Journal - Year 2014, Vol 14, Issue 1
Abstract
Objectives: Aflatoxin B1 (AFB1) is a naturally occurring carcinogenic and immunosuppressive compound. Tis study was designed to measure its toxic effects on human peripheral blood mononuclear cells (PBMC). Methods: Te study recruited 7 healthy volunteers. PBMC were isolated and cellular respiration was monitored using a phosphorescence oxygen analyser. Te intracellular caspase activity was measured by the caspase-3 substrate N-acetyl-asp-glu-val-asp-7-amino-4-methylcoumarin. Phosphatidylserine exposure and membrane permeability to propidium iodide (PI) were measured by flow cytometry. Results: Cellular oxygen consumption was inhibited by 2.5 µM and 25 µM of AFB1. Intracellular caspase activity was noted after two hours of incubation with 100 µM of AFB1. Te number of Annexin V-positive cells increased as a function of AFB 1 concentration and incubation time. At 50 µM, a signifcant number of cells became necrotic after 24 hours (Annexin V-positive and PI-positive). Conclusion: Te results show AFB1 is toxic to human lymphocytes and that its cytotoxicity is mediated by apoptosis and necrosis.
Authors and Affiliations
Suleiman Al-Hammadi| College of Medicine & Health Sciences, United Arab Emirates University, Al-Ain, United Arab Emirates, Farida Marzouqi| College of Medicine & Health Sciences, United Arab Emirates University, Al-Ain, United Arab Emirates, Aysha Al-Mansouri| College of Medicine & Health Sciences, United Arab Emirates University, Al-Ain, United Arab Emirates, Allen Shahin| College of Medicine & Health Sciences, United Arab Emirates University, Al-Ain, United Arab Emirates, Mariam Al-Shamsi| College of Medicine & Health Sciences, United Arab Emirates University, Al-Ain, United Arab Emirates, Eric Mensah-Brown| College of Medicine & Health Sciences, United Arab Emirates University, Al-Ain, United Arab Emirates, Abdul-Kader Souid| College of Medicine & Health Sciences, United Arab Emirates University, Al-Ain, United Arab Emirates
Keywords
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