The effect of dietary protein level on selected degradative enzymes activity of hepatocyte cellular subfractions in experimental mice
Journal Title: Animal Science Papers and Reports - Year 2014, Vol 32, Issue 3
Abstract
Studied was the activity of selected degradative enzymes of lysosomal, microsomal and cytoplasmic fraction of hepatocytes as affected by different protein levels in the diet of experimental mice. The control animals were fed with standard 16% protein level in the diet, while experimental animals with low (10%) or high (40%) protein diet. The animals were fed ad libitum. Changes in fractions depended on the kind of enzyme, cell fraction and protein level in examined diets. The high protein diet increased activity of six enzymes in the lysosomal fraction and decreased activity of β-Gal only, while the low protein diet caused an increase of activity of this fraction of seven enzymes. The 40% protein diet decreased the activity of nine enzymes in the microsomal fraction, the AcP did not change only. The 10% protein diet in this fraction decreased the activity of seven enzymes and increased activity of two enzymes. In the cytoplasmic fraction, the high protein diet decreased the activity of eight enzymes. Higher activity was observed only for AcP. The low protein diet caused a decrease in seven enzymes activity, and an increase of β-GlcUr activity only. The change of normal standard protein diet in animal feeding on its high or low-protein level occurred to be a significant factor disordering the biochemical homeostasis of the cell.
Authors and Affiliations
Bożena Witek, Wojciech Fronczyk, Artur Jóźwik, Marta Walczak, Agnieszka Kamińska, Adam Kołątaj
Keywords
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