The effect of light on the specimens of serum bilirubin in clinical laboratory
Journal Title: INTERNATIONAL JOURNAL OF CURRENT RESEARCH IN MEDICAL SCIENCES - Year 2016, Vol 2, Issue 8
Abstract
Background: Laboratories can influence the stability of bilirubin by the type of light used in the laboratory which has direct impact on the quantitative estimation of the bilirubin. Objective: To compare the levels of total and direct bilirubin in moderately hyperbilirubinemic to normobilirubinemic vs. Hyperbilirubinemic serum specimens before and after exposure to the room light. Materials and Methods: This was an experimental laboratory based study conducted in the department of clinical laboratory services (CLS), Birat Medical College and Teaching Hospital, Morang, Nepal. An early morning blood samples were collected from 50 patients who visited the CLS for their bilirubin and liver function test analysis. To evaluate the effect of light on the kinetics of bilirubin changes, each serum specimen was transferred into two microtubes with one tube completely enclosed in aluminum foil as a control in dark. Specimens were exposed to room light on a laboratory bench at room temperature for a set time interval of five hours. All specimens were assayed in duplicate directly from the microtubes for direct and total bilirubin before and after a set time interval of five hours by the diazo reaction. Result: In our present study, in normobilirubinemic / moderately hyperbilirubinemic specimen, measured values of total bilirubin decreased by an average of 55.9 % while the decline in total bilirubin for the hyperbilirubinemic specimen was smaller 32.4% when exposed to light but in contrary, direct bilirubin decreased by slightly more amounts, 47.4% and 49.7% respectively, for the normobilirubinemic / moderately hyperbilirubinemic specimen and hyperbilirubinemic specimen when exposed to light over a period of five hours. Conclusion: The practical consequences of this finding is that accurate measurement of bilirubin in specimens with normal or low bilirubin concentrations requires even greater care in the protection ofspecimens from light than for specimens with high bilirubin concentrations.
Authors and Affiliations
Santosh Kumar Deo1 , Abdul Haque Ansari1 Santosh Dev2
Keywords
Related Articles
- EP ID EP582384
- DOI -
- Views 178
- Downloads 1
A Review on Phytochemicals and their activit
Antioxidants are phytochemicals, vitamins and other nutrients that protect our cells from damage caused by free radicals. In vitro and in vivo studies have shown that antioxidants help prevent the free radical damage tha...
Characterization of Group B Streptococci from Rural Women Attending Primary Health Centre
The purpose of this study was to screen both pregnant and non-pregnant women in rural areas for GBS. A total of 30 vaginal samples were collected from patients attending Primary Health Care Centre, Cuddalore. The samples...
Electrocardiographic changes in acute stroke patients
Background: Cerebrovascular events are a major cause of morbidity and mortality worldwide. The primary reason is atherosclerotic disease of the cerebral arteries. Other causes are embolism, hemorrhage and hypo perfusion....
Siddha system of Medicine for treating Vathasthambam
The term sciatica describes a symptom pain along the sciatic nerve pathway rather than a specific condition, illness or disease. Some use it to mean any pain starting in the lower back and getting down the leg. Others us...
Diagnosis of Nodal Tubercluosis -by clinical and Histopathological correlation in pediatric age group (1-12 years)
Background: Cervical lymphadenopathy is a common clinical problem causing diagnostic dilemma. Various causes ranging from infections to autoimmune disorders are contributory. Hence it becomes essential to categorize them...