The effects of certain gasotransmitters inhibition on homocysteine acutely induced changes on rat cardiac acetylcholinesterase activity
Journal Title: Scripta Medica (Banja Luka) - Year 2019, Vol 50, Issue 3
Abstract
Background/Aim: Hyperhomocysteinaemia is linked to higher level of acetylcholinesterase (AChE) in brain, but there is insufficient information on influence of homocysteine (Hcy) and gasotransmitters on cardiac AChE. Thus, the aim of this study was to evaluate the influence of certain gasotransmitter inhibitors in Hcy-induced changes on rat cardiac AChE activity. Methods: Research was performed on 72 male Wistar albino rats distributed into 6 groups: 1) Control group – saline (1 ml 0.9 % NaCl ip); 2) DL-Hcy (8 mmol/kg ip DL homocysteine (DL-Hcy); 3) L-NAME (10 mg/kg ip N ω -Nitro-L-arginine methyl ester (L-NAME), inhibitor of NO production); 4) DL-PAG (50 mg/kg ip DL-propargylglycine (DL-PAG), inhibitor of H2S production); 5) DL-Hcy+L-NAME (8 mmol/ kg ip DL-Hcy + 10 mg/kg ip L-NAME); and 6) DL-Hcy+DL-PAG (8 mmol/kg ip DL-Hcy + 50 mg/kg ip DL-PAG). All tested substances were administered in a single dose, intraperitoneally, 60 minutes before animals’ sacrifice. AChE activity was measured in the rats’ cardiac tissue homogenate. Results: Administration of Hcy and L-NAME induced significant decrease in AChE activity compared with control condition. Administration of DL-PAG, DL-Hcy+LNAME and DL-Hcy+DL-PAG did not change AChE activity compared with the control group. Conclusion: The effects of acute Hcy administration on the cardiac AChE activity are partially mediated via interaction with tested gasotransmitters.
Authors and Affiliations
Marko Djuric, Slavica Mutavdzin, Dragana Loncar-Stojiljkovic, Sanja Kostic, Mirjana B. Colovic, Danijela Krstic, Vladimir Zivkovic, Dragan M. Djuric
Keywords
Related Articles
- EP ID EP673299
- DOI 10.5937/scriptamed50-22658
- Views 273
- Downloads 0
The effects of certain gasotransmitters inhibition on homocysteine acutely induced changes on rat cardiac acetylcholinesterase activity
Background/Aim: Hyperhomocysteinaemia is linked to higher level of acetylcholinesterase (AChE) in brain, but there is insufficient information on influence of homocysteine (Hcy) and gasotransmitters on cardiac AChE. Thus...
Quality of life in children with epilepsy
Children with epilepsy are a particularly sensitive part of population and require continuous monitoring by neuro-paediatricians, so that any behavioural and functional changes that occur during mental maturation from th...
Effects of the radial extracorporeal shock wave therapy (rESWT) in patients with calcific tendinopathy of the shoulder
Introduction: Calcific tendinopathy (CT) of the shoulder is frequently a painful condition that is occurring when there are calcium deposits in the tendons of the rotator cuff. There are many options for treatment starti...
Multimodal neural block analgesia versus morphine analgesia after elective knee surgery
Background: Total knee arthroplasty has become a standard for treatment of endstage knee osteoarthritis. Due to intense and complex knee innervation, there is a need to improve the anaesthetic/analgesic approach to such...
Dermal regeneration with MilliGraft® kit of nanofat: the micrograft of adipose tissue. A clinical assessment study
The simple filtration by means of the MilliGraft ® Kit of a disaggregated lipoaspirate allows to extract the class of cells defined as progentors with characteristics of adult stem cells present in the nanofat exclusivel...