The Effects Of Menadione On Rat Glial Cell Proliferation
Journal Title: Journal of Neurological Sciences-Turkish - Year 2007, Vol 24, Issue 1
Abstract
Background: Menadione inhibits cell growth of rodent and human cell lines. We reported that menadione had potential antiproliferative effect doses depend on both rat glioma (C6) and low passage human glioma cells in vitro. In this study, we examine menadione toxicity on primary glial cell culture cells in vitro. Material and Methods: Glial cells were obtained from 1-3 day old rat brain and cultured in humidified atmosphere of 5 % CO2, at 37 ºC in flask. The glial cells were seeded in 96 well plates and incubated for 24 hours. Then cells were exposed 10, 25, 50, 75 ,and 100 µM menadione doses. After 24 hours, menadione toxicity was measured by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, (MTT) assay.All statistical analyses were performed by one-way analysis of variance (ANOVA) and followed by Tukey’s multiple comparison tests. Results: When the compared to the control, 10 and 25 µM menadione doses did not shown any activity on glial cell proliferation (p>0.05). However, 50, 75 ,and 100 µM menadione doses reduced 49, 61, 63 % in glial cells viability respectively (p
Authors and Affiliations
VATAN, Selda KABADERE
Keywords
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