The key role of Kefir probiotic in enhancement the therapeutic action of mesenchymal stem cells in LPS- induced liver injury
Journal Title: Journal of Biomedical and Pharmaceutical Research - Year 2015, Vol 4, Issue 4
Abstract
Acute liver injury occurs after intraperitoneal injection of lipopolysaccharide (LPS). The release of pro-inflammatory cytokines and oxidative stress are both important in the pathogenesis of LPS-induced liver injury. This study investigated the key role of kefir probiotic in enhancement the therapeutic action of mesenchymal stem cell (MSCs) against LPS-induced liver injury. Sixty female rats were divided into 6 groups (10 rats each): Control group, LPS treated group, LPS + kefir, kefir + LPS + kefir, LPS + MSCs and kefir + LPS + MSCs +kefir. Hepatic function, cytokine level, MDA, GSH and the activity of antioxidant enzymes SOD and PON1 as well as the m RNA expression of NF-κB, iNOS and caspase-3 were monitored in rat's liver. Injection of LPS significantly increased ALT, AST, TNF-α in association with dramatic decrease of serum albumin and IL-10 levels. Oxidative stress evidenced by increased MDA in association with a significant decline of GSH content and the antioxidant enzymatic activity of SOD and PON1. Furthermore, the RNA expression of NF-κB, iNOS and caspase-3 were significantly up-regulated in liver tissue. Administration of kefir or MSCs alone significantly suppressed LPS-induced liver injury. The pre and co-treatment of kefir with MSCs potentiate the therapeutic action of MSCs. Pre and co-treatment of kefir probiotic with mesenchymal stem cell transplantation could be viable approach for the treatment of liver injury.
Authors and Affiliations
Nadia A. Eltablawy*| Biochemistry division, National organization for drug control and research (NODCAR), Laila A. Rashed| Medical Biochemistry, Faculty of Medicine, Cairo University
Keywords
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