The mRNA expression levels of uncoupling proteins 1 and 2 in mononuclear cells from patients with metabolic disorders: obesity and type 2 diabetes mellitus
Journal Title: Advances in Hygiene and Experimental Medicine - Year 2017, Vol 71, Issue
Abstract
<b>Introduction:</b> Type 2 diabetes mellitus (T2DM) and obesity are metabolic disorders whose major hallmark is insulin resistance. Impaired mitochondrial activity, such as reduced ratio of energy production to respiration, has been implicated in the development of insulin resistance. Uncoupling proteins (UCPs) are proton carriers, expressed in the mitochondrial inner membrane, that uncouple oxygen consumption by the respiratory chain from ATP synthesis. <br/><b>Aim:</b> The aim of the study was to determine transcriptional levels of <i>UCP1 </i>and <i>UCP2</i> in peripheral blood mononuclear cells (PBMCs) from patients with metabolic disorders: T2DM, obesity and from healthy individuals. <br/><b>Material/Methods:</b> The mRNA levels of <i>UCP1</i>, <i>UCP2</i> were determined by Real-Time PCR method using Applied Biosystems assays. <br/><b>Results:</b> The <i>UCP1</i> mRNA expression level was not detectable in the majority of studied samples, while very low expression was found in PBMCs from 3 obese persons. <i>UCP2</i> mRNA expression level was detectable in all samples. The median mRNA expression of <i>UCP2</i> was lower in all patients with metabolic disorders as compared to the controls (0.20+0.14 vs. 0.010+0.009, p=0.05). When compared separately, the differences of medians <i>UCP2</i> mRNA expression level between the obese individuals and the controls as well as between the T2DM patients and the controls did not reach statistical significance. <br/><b>Conclusions:</b> Decreased <i>UCP2</i> gene expression in mononuclear cells from obese and diabetic patients might contribute to the immunological abnormalities in these metabolic disorders and suggests its role as a candidate gene in future studies of obesity and diabetes.
Authors and Affiliations
Sona Margaryan, Agata Witkowicz, Anna Partyka, Levon Yepiskoposyan, Gayane Manukyan, Lidia Karabon
Keywords
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- EP ID EP229002
- DOI 10.5604/01.3001.0010.5386
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