The Neuroprotective Effect of Ursolic Acid Associated with Inflammation Factors: IL-10, IL β after the Induction of Severe Traumatic Brain Injury in Rats
Journal Title: The 1st Annual Meeting of Georgian Center for Neuroscience Research - Year 2020, Vol 2, Issue 20
Abstract
Introduction: Ursolic Acid (UA) is a Penta cyclic triterpene compound which Reduces cerebral edema and improves neurological deficits after TBI, leading to increased Nrf2 protein and increased expression of reductase-1 and cooxygenase enzymes, which is suggested to play a neuroprotective role. Therefore, in this study, we investigated the effects of neuroprotective UA after induction of brain inflammation in rats. Materials and methods: The male Albino Westar rats received different doses of Ursolic Acid (25, 50, 100 mg/kg, I.P) UA .All animals were intubated before surgery. In the TBI groups, diffuse TBI was induced by Marmarou method using a TBI induction device. The severe TBI was induced using a weight 450gr in the sham groups, all stages of induction of TBI were performed except dropping weight on the head. The disruption of Blood brain- barrier (BBB) was evaluated 6h Post-TBI. The neurologic score (VCS) and brain water content, the beam-walk –balance task (WB) were determined before trauma, on trauma time(D0), and 1Day (D1) and 2 Days (D2) and 3 Days (D3) After TBI anaesthetized animals CSF collection from cisterna magna and then were sacrificed and the brain was removed and then analysis MMP-9 with Elisa assay. Results: Our results showed that traumatic brain injury leads to significant brain edema and disrupt of blood brain-barrier and neurological defect and vestibulomotor dysfunction in the rats brain and decreases MMP9 in CSF. UA (25,50mg/kg) could attenuate brain edema, improve BBB and vestibulomotor dysfunction in compare with TBI control group (P<0.001) but in 25 mg/kg dose results were better. Discussion: Our findings showed that UA has a prominent role in TBI outcome’s and perhaps protects neurons through modulating inflammatory and antioxidant pathways so reduces the level of IL-1 β and increases the level of IL-10 in CSF.
Authors and Affiliations
Fakhroddin Alemi, Ali Siahposht-Khachaki, Ali Alinejad-Naddafi, Davood Farzin
Keywords
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- EP ID EP677916
- DOI 10.29088/GCNR-2020.07
- Views 328
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