The Protective Effects and Mechanism of Mulberry Leaf Flavonoids on Type 2 Diabetic Nephropathy Mice
Journal Title: Journal of Experimental and Clinical Application of Chinese Medicine - Year 2024, Vol 5, Issue 4
Abstract
Purpose: This study investigated the protective effects and mechanism of mulberry leaf flavonoids on type 2 diabetic nephropathy (DN) in mice. Methods: The DPPH free radical scavenging activity of mulberry leaf flavonoids was measured in vitro. To establish a type 2 DN model, male ICR mice were induced with streptozotocin (STZ) after a high-fat diet regimen. The mice were then divided into groups: a normal control group, a DN model group (DM), a positive control group receiving 200 mg/kg metformin (Met group), and a treatment group receiving 1 g/kg mulberry leaf flavonoids (FM group). Both Met and FM groups received daily intragastric administration for 4 weeks, while the DM and control groups were given equivalent volumes of normal saline. Fasting blood glucose levels were recorded regularly during the intervention. After the treatment period, serum creatinine (CRE), blood urea nitrogen (BUN), superoxide dismutase (SOD) activity, malondialdehyde (MDA) levels, and renal glutathione peroxidase (GPx) activity were measured. Additionally, kidney histopathological changes and glycogen deposition were evaluated by HE and PAS staining, while Nrf2 and HO-1 expression in kidney tissue was assessed through immunohistochemistry. Results: Mulberry leaf flavonoids effectively scavenged free radicals, significantly reduced blood glucose levels, CRE, BUN, and MDA in serum and kidney tissues, and increased SOD and GPx activities. Histopathological improvements in kidney and pancreatic tissues were observed, alongside increased Nrf2 and HO-1 expression. Conclusion: Mulberry leaf flavonoids ameliorate renal damage in DN mice, potentially through oxidative stress reduction and activation of the Nrf2/HO-1 signaling pathway.
Authors and Affiliations
Huajie Hu, Yanna Guo, Leifang Zhang, Ke Li
Keywords
Related Articles
- EP ID EP754223
- DOI -
- Views 33
- Downloads 0
Rhizoma Zedoariae Inhibits G-CSF-Induced MDSCs-like Differentiation of THP-1 Cells
Objective: Rhizoma Zedoariae is widely used in the treatment of solid tumors in China, but there has been no deep research on its role in the formation of timmunosuppressive environments mediated by myeloid-derived suppr...
Pharmacological Activities of a Chinese Herb, Glycine tomentella (I-Tiao-Gung): A Systematic Review
I-Tiao-Gung, Glycine temetella Hayata, has been used as a traditional herbal medicine for pain relief. This review provides science-based evidences that support the traditional concept. The 95 % ethanol extract of I-Tiao...
Effect of Jinhuahecha—A Fermented Green Tea on DSS-Induced Ulcerative Colitis in Mice and Its Preliminary Pharmacological Mechanism
Background: Ulcerative colitis (UC) is an inflammatory bowel disease. Jinhuahecha (JHHC)-a fermented green tea is obtained by micro-fermentation of Anji white tea (AJWT) inoculated with high-purity "E. cristatum". Purpos...
Dynamic Changes of Phytohormone Concentrations during Prunella vulgaris L. Development and Impact of Rosmarinic Acid Accumulation through Exogenous ABA Application
Background: Plant hormones not only participate in regulating the growth and development process of plants, but also play an important regulatory role in the synthesis of secondary metabolites. Prunella vulgaris L. (P. v...
Exploring the Mechanism of "Radix Bupleuri-Paeoniae Radix Alba" in the Treatment of Post-Stroke Depression Based on Network Pharmacology and Molecular Docking
Purpose: To explore the mechanism of “Radix Bupleuri-Paeoniae Radix Alba” in the treatment of post-stroke depression through network pharmacology and molecular docking. Methods: The active ingredients and targets of “Ra...