The Role of N-Acetylcysteine Supplementation on the Oxidative Stress Levels, Genotoxicity and Lineage Commitment Potential of Ex Vivo Murine Haematopoietic Stem/Progenitor Cells
Journal Title: Sultan Qaboos University Medical Journal - Year 2018, Vol 18, Issue 2
Abstract
Objectives: Te ex vivo maintenance of haematopoietic stem/progenitor cells (HSPCs) is crucial to ensure a sufcient supply of functional cells for research or therapeutic applications. However, when exposed to reactive oxygen species (ROS) in a normoxic microenvironment, HSPCs exhibit genomic instability which may diminish their quantity and quality. Tis study aimed to investigate the role of N-acetylcysteine (NAC) supplementation on the oxidative stress levels, genotoxicity and lineage commitment potential of murine haematopoietic stem/progenitor cells (HSPCs). Methods: Tis study was carried out at the Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia, between June 2016 and July 2017. Bone marrow cells were isolated from nine mice and cultured in a growth medium. Various concentrations of NAC between 0.125–2 µM were added to the culture for 48 hours; these cells were then compared to non-supplemented cells harvested from the remaining three mice as the control group. A trypan blue exclusion test was performed to determine cell viability, while intracellular ROS levels and genotoxicity were determined by hydroethidine staining and comet assay, respectively. Te lineage commitment potential of erythroid, myeloid and pre-B-lymphoid progenitor cells was evaluated via colony-forming cell assay. Results: NAC supplementation at 0.25, 0.5 and 2 µM signifcantly increased cell viability (P <0.050), while intracellular ROS levels signifcantly decreased at 0.25 and 0.5 µM (P <0.050). Moreover, DNA damage was signifcantly reduced at all NAC concentrations (P <0.050). Finally, the potential lineage commitment of the cells was not signifcantly affected by NAC supplementation (P >0.050). Conclusion: Te fndings of this study indicate that NAC supplementation may potentially overcome the therapeutic limitations of ex vivo-maintained HSPCs.
Authors and Affiliations
Zariyantey A. Hamid, Hui Y. Tan, Paik W. Chow, Khairul A. W. Harto, Chin Y. Chan, Jamaludin Mohamed
Keywords
Related Articles
- EP ID EP29157
- DOI -
- Views 366
- Downloads 8
Genetic Counseling Practice Advanced Concepts and Skills
5th International Obstetrics & Gynecology Conference, Sultan Qaboos University, Sultanate of Oman 0-3 December 2007
The Disinfecting Potential of Contact Lens Soutions used by Sultan Qaboos University Students
Objectives: Tis study aimed to determine the disinfecting potential of some contact lens solutions used by some university students in Oman. Methods: Tis work was carried out from January to June 2010 in the Department...
Risk Factors Associated with Denture Stomatitis in Healthy Subjects Attending a Dental School in Southeast Iran
Objectives: Tere is scant information regarding the multifactorial aetiology of denture stomatitis (DS) in the world and, despite its frequency, associated factors are not completely understood. Te aim of this study wa...
Pyogenic Granuloma and Arteriovenous Malformation
A44-year-old male patient presented to the Kasturba Medical College Hospital, Manipal, India, in 2018 with a two-month history of a repeatedly bleeding, non-healing nodule over the tip of his left thumb following minor...