The Use of Bacillus Amyloliquefaciens to Control of Sclerotinia Stem Rot (Sclerotinia Sclerotiorum) of Cucumber
Journal Title: International Journal of Farming and Allied Sciences - Year 2013, Vol 2, Issue 22
Abstract
Sclerotinia sclerotiorum (Lib.) de Bary is a soil-borne pathogen capable of infecting more than 400 host plants worldwide. Antagonistic rhizobacteria, more specifically certain species of Bacillus, are known to control of fungal root diseases of agronomic crops (Ashwini and Srividya, 2013) . In this study, 182 bacteria were isolated of cucumber rhizosphere and screened as potential biological control agents against S. sclerotiorum in vitro condition. Morphology, physiological and biochemical tests and 16S rDNA analysis demonstrated that Bacillus amyloliquefaciens significantly reduce the mycelial growth of S. sclerotiorum by 72.04% . Non-volatile compounds of 3 isolates showed noticeable inhibition zone (>36%) against S.sclerotiorum, whereas volatile compounds of 7 isolates could prevent more than 30% of the mycelial growth of the fungus. All strains except of FE1 promoted significantly plant growth in comparision to non-treated control. The population density on roots of plants treated with PE and AE reached to 8.40 and 7.81 log10 CFU g−1 root respectively. The use of bacteria as a soil treatment (PE 69.6%) was more effective to suppress the disease than those as a seed treatment (PE 62.3%). These results indicate that bacterial species used in this study could be used in the control of S. sclerotiorum.
Authors and Affiliations
Sharie Rostami, Mojdeh Maleki, Dariush Shahriari
Keywords
Related Articles
- EP ID EP32430
- DOI -
- Views 324
- Downloads 0
Response of some wheat genotypes to drought stress by indices of drought resistance
In order to identify the effective traits of performance and the best index of drought resistance on bread wheat in cold regions of Ardabil province, an experiment was conducted on 10 wheat crop in a randomized complet...
The nature and origin of sand dunes in Kashan ERG, Iran
Wind erosion is a process of wind-forced movement of soil particles. This process has the distinct phases of particle entrainment, transport and deposition. Because Iran locate in area that is sensitive about geologica...
Stomata and trichrome performances of four radish Raphanus sativus L. var. sativus cultivars grown in controlled cabinet under varying temperatures and irrigation levels
Topsi, Famox F1, Corox F1, and Altox F1 radish cultivars were grown in controlled 20 and 12oC cabinets and they were subjected to 0, 33, 66 and 100% depletion of peat moss available water capacity (AWC). The objective...
Improvement of the eating and cooking qualities of rice: a review
Yet rice feeds the largest number of people in the world. At the same time rice is very flexible and grows practically all over the world. The demand for superior grain quality represents a major issue in rice (Oryza s...
Investigation of the Affection of Some Gums on the Low Fat Sausage Properties
Today reduction of fat in high fat meat products and producing more healthy food stuff is one of the important policies in inhibiting of dangerous diseases. Among food additives, fat replacers are of more interested by...