Thermo-tolerance characterization and bioassay of selected entomopathogenic fungal (EPF) isolates on larva of Spodoptera litura Fab.
Journal Title: Journal of Applied and Natural Science - Year 2019, Vol 11, Issue 1
Abstract
Entomopathogenic fungi viz. Metarizhium anisoplae and Beauveria bassiana are well characterized in respect to pathogenicity to several insects and have been used for biological control of agriculture pests worldwide. Despite their potential, several factors like low resistance to elevated temperature have hindered widespread adoption of fungi as part of biological control regimes. In this study, five isolates of B. bassiana (Bb 01, Bb 02, Bb 03, Bb 04 and Bb 05) and two isolates of M. anisoplae (Ma 01 and Ma 02) were evaluated for thermotolerance capacity and bioefficacy against third instar of Spodoptera litura. Among the isolates studied, Bb 04 recorded the higher temperature tolerance up to 34oC with mean spore germination of 35.00 percent and mean dry mycelial weight of 443.38 mg. Further, in the bioassay studies, Bb 04 caused significantly (@ P<0.01%) higher percent mortality of S. litura larva at 1×108 spores ml-1 on fifth day (80.00 %) with LT50 value of 4.08 days and LC50 value of 2.07×104 conidia ml-1 at five days. Investigation results indicated that BB 04 is a suitable EPF isolate for management of S. litura where higher temperature prevails.
Authors and Affiliations
S. Deepak, A. G. Sreenivas, Basavaraj Kalmath, S. G. Hanchinal, K. T. Vendan, S. S. Patil
Keywords
Related Articles
- EP ID EP485148
- DOI 10.31018/jans.v11i1.2007
- Views 100
- Downloads 0
Determination of anticholinesterase and antioxidant activities of methanol and water extracts of leaves and fruits of Chamaerops humilis L.
The present study was carried out to investigate the antioxidant activity of the water and methanol extracts of leaves and fruits extracts of Chamaerops humilis L. by using ABTS cation radicals and cupric reducing antiox...
Stability factor and heterosis for yield and yield determinants over environments in Indian rapeseed, Brassica rapa var. yellow sarson
Forty-four yellow sarson hybrids developed by line x testers mating design (3 pistillate lines x 9 male parents) were studied along with parents and a standard check for heterosis of yield determinant characters in two e...
In vivo evaluation of ziram induced acute toxicity on pathomorphology of broiler chicken
Fungicides are usually used in agriculture and often find their way in poultry feed. Therefore, a study was undertaken to study the in vivo effect of one such fungicide (ziram) induced intoxication on pathomorphology of...
Analysis of seed protein diversity in Cicer arietinum L. genotypes with different seed coat colour using SDS-PAGE
SDS-PAGE technique was used for the study of seed protein polymorphism among three genotypes of Cicer arietinum with different seed coat colour. A total of 24 polypeptide bands were recorded. Out of these 20 were common...
Isolation and characterization of insoluble inorganic phosphate solubilizer rice rhizosphere strain Enterobacter cloacae BAU3
The objective of the present study was to isolate and characterize most efficient phos-phate solubilizing bacteria (PSB) from rice rhizosphere. The study was carried out during the Kharif season’2018 at Department of Soi...