To determine antifungal susceptibility of dermatophyte isolates in a tertiary care hospital using microdilution method: A prospective cohort study
Journal Title: Innovations in Pharmaceuticals and Pharmacotherapy (IPP) - Year 2018, Vol 6, Issue 2
Abstract
Aim: The aim of the study was to standardize in vitro antifungal susceptibility testing by microbroth dilution method to find out the minimum inhibitory concentration (MIC) of fluconazole, ketoconazole, itraconazole, terbinafine, and griseofulvin fungal isolates of skin, hair, and nail. Materials and Mehods: Various samples were collected from patients with clinically diagnosed dermatophytosis. Skin scrapings, hair, and nail were collected from 170 patients. Results: The antifungal drugs such as fluconazole (0.5–0.16 µg/ml), ketoconazole (0.03–0.5 µg/ml), itraconazole (0.007–0.25 µg/ ml), terbinafine (32–0.0313 µl/ml), and griseofulvin (0.03–0.25 µg/ml) these water-insoluble drugs were incorporated in dissolved in dimethyl sulfoxide. The MIC range, MIC 50, and MIC 90 for the drug griseofulvin were found to be 0.03–0.25, 0.06, and 0.12, respectively, for ketoconazole were found to be 0.03–0.5, 0.12, and 0.5, respectively, for the drug fluconazole were found to be 1–32, and 1, respectively, itraconazole were found to be 0.007–0.06, 0.03, and 0.06, respectively, and for terbinafine were found to be 0.007–0.06, 0.015, and 0, respectively. This technique was found to be reliable, cost-effective, and easy to perform with consistent results. Conclusion: Further, results concluded that the itraconazole showed a higher MIC value when compared to other antifungal drugs.
Authors and Affiliations
M. Suganth
Keywords
Related Articles
- EP ID EP570123
- DOI -
- Views 98
- Downloads 0
Process development, evaluation and controlling of parameters during formulation development of Loratadine as an ODT by QBD concept
Quality by design (QbD) refers to an advanced approach toward drug development. QbD is a vital part of the modern approach to pharmaceutical quality. There is much confusion among pharmaceutical scientists in generic dru...
Intravenous drug incompatibility in intensive care units - A comprehensive review
In intensive care unit, intravenous (IV) therapy is preferred over oral therapy for a faster therapeutic action. IV therapy is complex and error prone, requiring strategies to reduce the risk and complications. Once inje...
Formulation and evaluation of novel herbal antidiabetic transdermal patch
Aim: The aim of the present study was to formulate and evaluate the Transdermal Drug Delivery System of an antidiabetic herbal drug. And to increase the efficacy and to improve the patience compliance of the herbal medic...
To determine antifungal susceptibility of dermatophyte isolates in a tertiary care hospital using microdilution method: A prospective cohort study
Aim: The aim of the study was to standardize in vitro antifungal susceptibility testing by microbroth dilution method to find out the minimum inhibitory concentration (MIC) of fluconazole, ketoconazole, itraconazole, ter...
Development and evaluation of some novel polyherbal anti-inflammatory and analgesic formulations of some Indian spices
Inflammation is linked with a broad range of infectious and non-infectious diseases; hence, also considered on root of the all diseases. The crucial role of inflammatory processes makes possible evaluation research focus...